ICC/IF
Phospho-IP3 Receptor (Ser1756) Antibody staining Hela cells(heat shock treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Product Name | Rabbit polyclonal antibody to Phospho-IP3 Receptor (Ser1756) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from RAT IP3 Receptor(P29994) around the phosphorylation site of Ser1756. This site is equivalent to Ser1764 in the human IP3 receptor sequence. |
| Modification | p-Ser1756 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF |
| ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ITPR1 |
|---|---|
| Gene Synonyms | ACV CLA4 IP3R IP3R1 SCA15 SCA16 SCA29 INSP3R1 PPP1R94 |
| Gene Full Name | inositol 1,4,5-trisphosphate receptor type 1 |
| Gene Summary | This gene encodes an intracellular receptor for inositol 1,4,5-trisphosphate. Upon stimulation by inositol 1,4,5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Mutations in this gene cause spinocerebellar ataxia type 15, a disease associated with an heterogeneous group of cerebellar disorders. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Nov 2009] |
| Alternative Names | 4 5-trisphosphate receptor 5-trisphosphate receptor type 1 DKFZp313E1334 DKFZp313N1434 inositol 1 4 5 triphosphate receptor type 1 Inositol 1 4 5 trisphosphate Receptor Type 1 Inositol 1 InsP3R1 IP3 IP3 receptor IP3 receptor isoform 1 IP3R 1 IP3R IP3R1 ITPR 1 Itpr1 ITPR1_HUMAN SCA15 SCA16 SCA29 Type 1 inositol 1 4 5 trisphosphate receptor Type 1 inositol 1 Type 1 InsP3 receptor |
| Molecular Weight(MW) | 314 kDa |
| Cellular Localization | Endoplasmic reticulum membrane>Multi-pass membrane protein. Cytoplasmic vesicle>Secretory vesicle membrane>Multi-pass membrane protein. Cytoplasm>Perinuclear region. |
ICC/IF
Phospho-IP3 Receptor (Ser1756) Antibody staining Hela cells(heat shock treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Application Notes | ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
