IHC
Phospho-SLP76 (Ser376) Antibody at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.ICC/IF
Phospho-SLP76 (Ser376) Antibody staining A549 cells(H2O2 treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Product Name | Rabbit polyclonal antibody to Phospho-SLP76 (Ser376) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human SLP76 around the phosphorylation site of Ser376. |
| Modification | p-Ser376 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC |
| ICC/IF:1:100-1:500 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LCP2 |
|---|---|
| Gene Synonyms | IMD81 SLP76 SLP-76 |
| Gene Full Name | lymphocyte cytosolic protein 2 |
| Gene Summary | This gene encodes an adapter protein that acts as a substrate of the T cell antigen receptor (TCR)-activated protein tyrosine kinase pathway. The encoded protein associates with growth factor receptor bound protein 2, and is thought to play a role TCR-mediated intracellular signal transduction. A similar protein in mouse plays a role in normal T-cell development and activation. Mice lacking this gene show subcutaneous and intraperitoneal fetal hemorrhaging, dysfunctional platelets and impaired viability. [provided by RefSeq, Nov 2016] |
| Alternative Names | 76 kDa tyrosine phosphoprotein CG8697 LCP 2 LCP2 LCP2_HUMAN Lymphocyte cytosolic protein 2 SH2 domain containing leukocyte protein 76 KD SH2 domain containing leukocyte protein of 76kD SH2 domain containing leukocyte protein of 76kDa SH2 domain-containing leukocyte protein of 76 kDa SLP 76 SLP 76 tyrosine phosphoprotein SLP-76 SLP-76 tyrosine phosphoprotein SLP76 SLP76 tyrosine phosphoprotein |
| Molecular Weight(MW) | 60 kDa(Observed band size: 65-85kDa) |
| Cellular Localization | Cytoplasm. |
IHC
Phospho-SLP76 (Ser376) Antibody at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
ICC/IF
Phospho-SLP76 (Ser376) Antibody staining A549 cells(H2O2 treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Application Notes | ICC/IF:1:100-1:500 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
