WB
HEK-293 cells were subjected to SDS PAGE followed by western blot with PELO antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.WB
Various cells were subjected to SDS PAGE followed by western blot with PELO antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.WB
Various lysates were subjected to SDS PAGE followed by western blot with PELO antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using PELO antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using PELO antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using PELO antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-PELO (IP: 4ug; Detection:1:500) with HEK-293 cells lysate 1200ug.ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PELO antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).| Product Name | PELO Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human PELO protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:2000-1:12000 IHC:1:250-1:1000 ICC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PELO |
|---|---|
| Gene Synonyms | CGI-17 PRO1770 |
| Gene Full Name | pelota mRNA surveillance and ribosome rescue factor |
| Gene Summary | This gene encodes a protein which contains a conserved nuclear localization signal. The encoded protein may have a role in spermatogenesis, cell cycle control, and in meiotic cell division. [provided by RefSeq, Jul 2008] |
| Alternative Names | CGI 17 CGI-17 hPelota PRO1770 Protein Dom34 homolog |
| Molecular Weight(MW) | 43 kDa(Observed band size: 43-45 kDa) |
| Cellular Localization | Cytoplasm. |
WB
HEK-293 cells were subjected to SDS PAGE followed by western blot with PELO antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB
Various cells were subjected to SDS PAGE followed by western blot with PELO antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB
Various lysates were subjected to SDS PAGE followed by western blot with PELO antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using PELO antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using PELO antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using PELO antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-PELO (IP: 4ug; Detection:1:500) with HEK-293 cells lysate 1200ug.
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PELO antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).| Application Notes | WB:1:2000-1:12000 IHC:1:250-1:1000 ICC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
