WB
Western blot analysis of Gephyrin/GPHN using anti-Gephyrin/GPHN antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Hela whole cell lysates, Lane 2: HepG2 whole cell lysates, Lane 3: A549 whole cell lysates, Lane 4: COS-7 whole cell lysates, Lane 5: Jurkat whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse kidney tissue lysates, Lane 9: mouse lung tissue lysates, Lane 10: Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Gephyrin/GPHN antigen affinity purified polyclonal antibodyat a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
Gephyrin/GPHN was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Gephyrin/GPHN Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
Gephyrin/GPHN was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Gephyrin/GPHN Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
Gephyrin/GPHN was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Gephyrin/GPHN Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
Gephyrin/GPHN was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-Gephyrin/GPHN Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Rabbit polyclonal antibody to Gephyrin/GPHN |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human Gephyrin/GPHN recombinant protein (Position: K148-L736). Human GPHN shares 94.5% and 96.7% amino acid (aa) sequence identity with mouse and rat GPHN, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 ICC/IF:1:50-1:400 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GPHN |
|---|---|
| Gene Synonyms | GPH, GEPH, HKPX1, GPHRYN, MOCODC |
| Gene Full Name | gephyrin |
| Gene Summary | This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known. [provided by RefSeq, Jul 2008] |
| Alternative Names | Domain E GEPH gephyrin GPH GPHN GPHRYN KIAA1385 MPT adenylyltransferase MPT Mo transferase |
| Molecular Weight(MW) | 80 kDa(Observed band size: 93 kDa) |
| Cellular Localization | Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Postsynaptic cell membrane, Synapse. |
WB
Western blot analysis of Gephyrin/GPHN using anti-Gephyrin/GPHN antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Hela whole cell lysates, Lane 2: HepG2 whole cell lysates, Lane 3: A549 whole cell lysates, Lane 4: COS-7 whole cell lysates, Lane 5: Jurkat whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse kidney tissue lysates, Lane 9: mouse lung tissue lysates, Lane 10: Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Gephyrin/GPHN antigen affinity purified polyclonal antibodyat a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
Gephyrin/GPHN was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Gephyrin/GPHN Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
Gephyrin/GPHN was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Gephyrin/GPHN Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
Gephyrin/GPHN was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Gephyrin/GPHN Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
Gephyrin/GPHN was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-Gephyrin/GPHN Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 ICC/IF:1:50-1:400 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
