WB
Western blot analysis of extracts from HepG2 cells, using PDZK1 Antibody. The lane on the left was treated with blocking peptide.ICC/IF
PDZK1 Antibody staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Product Name | Rabbit polyclonal antibody to PDZK1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human PDZK1, corresponding to a region within C-terminal amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| ICC/IF:1:100-1:500 WB:1:500-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDZK1 |
|---|---|
| Gene Synonyms | CAP70 CLAMP PDZD1 NHERF3 NHERF-3 |
| Gene Full Name | PDZ domain containing 1 |
| Gene Summary | This gene encodes a PDZ domain-containing scaffolding protein. PDZ domain-containing molecules bind to and mediate the subcellular localization of target proteins. The encoded protein mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. Single nucleotide polymorphisms in this gene may be associated with metabolic syndrome, and overexpression of this gene may play a role in drug resistance of multiple myeloma. Pseudogenes of this gene are located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011] |
| Alternative Names | 1700023D20Rik 2610507N21Rik 4921513F16Rik AI267131 AI314638 AL022680 C terminal linking and modulating protein CAP70 CFTR associated protein of 70 kDa CFTR associated protein 70-KD CFTR-associated protein of 70 kDa CLAMP D3Ertd537e Dietary Pi-regulated RNA-1 Diphor-1 mPDZK1 Na(+)/H(+) exchange regulatory cofactor NHE-RF3 Na(+)/H(+) exchanger regulatory factor 3 Na/Pi cotransporter C-terminal-associated protein 1 Na/Pi cotransporter C-terminal-associated protein NaPi Cap1 NaPi-Cap1 NaPiCap1 NHERF 3 NHERF-3 NHERF3 NHRF3_HUMAN OTTHUMP00000015572 PDZ domain containing 1 PDZ domain containing protein 1 PDZ domain-containing protein 1 PDZD1 PDZK1 Sodium hydrogen exchanger regulatory factor 3 Sodium-hydrogen exchanger regulatory factor 3 |
| Molecular Weight(MW) | 57 kDa(Observed band size: 57 kDa, 90 kDa) |
| Cellular Localization | Membrane>Peripheral membrane protein. Cell membrane. |
WB
Western blot analysis of extracts from HepG2 cells, using PDZK1 Antibody. The lane on the left was treated with blocking peptide.
ICC/IF
PDZK1 Antibody staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Application Notes | ICC/IF:1:100-1:500 WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
