WB
Western blot analysis of anti- SUSD6 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Use rabbit anti- SUSD6 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.FC
Flow Cytometry analysis of Jurkat cells using anti-SUSD6 antibody. Overlay histogram showing Jurkat cells stained with anti-SUSD6 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUSD6 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to SUSD6 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human SUSD6 recombinant protein (Position: L44-A303). Human SUSD6 shares 90% amino acid (aa) sequence identity with mouse SUSD6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCWB |
| WB:1:500-1:2000 FC:1:50-1:200 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SUSD6 |
|---|---|
| Gene Synonyms | DRAGO KIAA0247 |
| Gene Full Name | sushi domain containing 6 |
| Gene Summary | Involved in DNA damage response. Predicted to be located in membrane. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | SUSD6 DRAGO KIAA0247 Sushi domain-containing protein 6 Drug-activated gene overexpressed protein |
| Molecular Weight(MW) | 32 kDa(Observed band size:35 kDa) |
| Cellular Localization | Membrane |
WB
Western blot analysis of anti- SUSD6 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Use rabbit anti- SUSD6 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.
FC
Flow Cytometry analysis of Jurkat cells using anti-SUSD6 antibody. Overlay histogram showing Jurkat cells stained with anti-SUSD6 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUSD6 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
