WB
WB result of BRD8 antibody (1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BRD8 transfected HeLa cells.WB
Various lysates were subjected to SDS PAGE followed by western blot with BRD8 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using BRD8 antibody at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BRD8 antibody at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).| Product Name | Featured Product KD/KO Validated BRD8 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human BRD8 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:6000 IHC:1:50-1:500 ICC/IF:1:400-1:1600 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BRD8 |
|---|---|
| Gene Synonyms | SMAP p120 SMAP2 |
| Gene Full Name | bromodomain containing 8 |
| Gene Summary | The protein encoded by this gene interacts with thyroid hormone receptor in a ligand-dependent manner and enhances thyroid hormone-dependent activation from thyroid response elements. This protein contains a bromodomain and is thought to be a nuclear receptor coactivator. Multiple alternatively spliced transcript variants that encode distinct isoforms have been identified. [provided by RefSeq, Jul 2014] |
| Alternative Names | TrCP120 SMAP2 SMAP p120 Bromodomain-containing protein 8 |
| Molecular Weight(MW) | 120 kDa(Observed band size: 120-170 kDa) |
| Cellular Localization | Nucleus. |
WB
WB result of BRD8 antibody (1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BRD8 transfected HeLa cells.
WB
Various lysates were subjected to SDS PAGE followed by western blot with BRD8 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using BRD8 antibody at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BRD8 antibody at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).| Application Notes | WB:1:1000-1:6000 IHC:1:50-1:500 ICC/IF:1:400-1:1600 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
