WB
Western blot analysis of Iba1 on different lysates with Chicken anti-Iba1 antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat anti-Chicken IgY - HRP Secondary Antibody at 1/10,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of C6 cells labeling Iba1 with Chicken anti-Iba1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Chicken anti-Iba1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Chicken IgY (647) was used as the secondary antibody at 1/250 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.FC
Flow cytometric analysis of THP-1 cells labeling Iba1. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 750 conjugate-Goat anti-Chicken IgY Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IF-F
Species: Mouse, Site: Cerebellum, Sample: Frozen sectio, Antibody concentration: 1/500 (Iba1,green); 1/500 (MAG,red), Antigen retrieval: Not required,IF-F
Species: Rat, Site: Spleen, Sample: Frozen section, Antibody concentration: 1/500 (Iba1,green); 1/1,000 (F4/80,red), Antigen retrieval: Not required.| Product Name | Iba1 Recombinant Antibody - Chicken IgY (Chimeric) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within N-terminal human Iba1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgY |
| Tested Applications | FCICC/IFIF-FWB |
| IF-F:1:500 WB:1:5000 ICC/IF:1:100 FC:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | AIF1 |
|---|---|
| Gene Synonyms | IBA1 IRT1 AIF-1 IRT-1 |
| Gene Full Name | allograft inflammatory factor 1 |
| Gene Summary | This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016] |
| Alternative Names | AIF 1 antibody AIF-1 antibody Aif1 antibody AIF1 protein antibody AIF1_HUMAN antibody Allograft inflammatory factor 1 antibody Allograft inflammatory factor 1 splice variant G antibody allograft inflammatory factor-1 splice variant Hara-1 antibody balloon angioplasty responsive transcription antibody BART 1 antibody AIF 1 antibody AIF-1 antibody Aif1 antibody AIF1 protein antibody AIF1_HUMAN antibody Allograft inflammatory factor 1 antibody Allograft inflammatory factor 1 splice variant G antibody allograft inflammatory factor-1 splice variant Hara-1 antibody balloon angioplasty responsive transcription antibody BART 1 antibody G1 antibody G1 putative splice variant of allograft inflamatory factor 1 antibody IBA 1 antibody IBA1 antibody interferon gamma responsive transcript antibody Interferon responsive transcript 1 antibody interferon responsive transcript factor 1 antibody Ionized calcium binding adapter molecule 1 antibody Ionized calcium-binding adapter molecule 1 antibody ionized calcium-binding adapter molecule antibody IRT 1 antibody IRT1 antibody Microglia response factor antibody MRF1 antibody Protein g1 antibody |
| Molecular Weight(MW) | 17 kDa |
| Cellular Localization | Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, Cell projection, phagocytic cup. |
WB
Western blot analysis of Iba1 on different lysates with Chicken anti-Iba1 antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat anti-Chicken IgY - HRP Secondary Antibody at 1/10,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of C6 cells labeling Iba1 with Chicken anti-Iba1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Chicken anti-Iba1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Chicken IgY (647) was used as the secondary antibody at 1/250 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
FC
Flow cytometric analysis of THP-1 cells labeling Iba1. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 750 conjugate-Goat anti-Chicken IgY Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IF-F
Species: Mouse, Site: Cerebellum, Sample: Frozen sectio, Antibody concentration: 1/500 (Iba1,green); 1/500 (MAG,red), Antigen retrieval: Not required,
IF-F
Species: Rat, Site: Spleen, Sample: Frozen section, Antibody concentration: 1/500 (Iba1,green); 1/1,000 (F4/80,red), Antigen retrieval: Not required.| Application Notes | IF-F:1:500 WB:1:5000 ICC/IF:1:100 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
