WB
Western blot analysis of Periostin on different lysates with Rabbit anti-Periostin antibody at 1/5,000 dilution. Lane 1: Mouse skin tissue lysate, Lane 2: Mouse spleen tissue lysate, Lane 3: Mouse skeletal muscle tissue lysate, Lane 4: Mouse stomach tissue lysate, Low expression: Mouse spleen, Mouse skeletal muscle. Lysates/proteins at 30 µg/Lane. Exposure time: 43 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 1/5,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP , 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.IHC
Species: Mouse, Tissue: Skin, Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Species: Mouse, Tissue: Stomach, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IF-F
Species: Mouse, Tissue: Stomach, Sample: Frozen section, Antigen retrieval: Not required Wash buffer: 1× TBST, Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 1.5 hours at room temperature.| Product Name | Periostin Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse Periostin aa 1-838. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIHCWB |
| WB:1:5000 IHC:1:1000 IF-F:1:500 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | POSTN |
|---|---|
| Gene Synonyms | PN OSF2 OSF-2 PDLPOSTN |
| Gene Full Name | periostin |
| Gene Summary | This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing, and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015] |
| Alternative Names | Fasciclin-I like antibody MGC119510 antibody MGC119511 antibody OSF 2 antibody OSF-2 antibody OSF2 antibody Osteoblast specific factor 2 (fasciclin I like) antibody Osteoblast specific factor 2 antibody Osteoblast specific factor antibody Osteoblast-specific factor 2 antibody Fasciclin-I like antibody MGC119510 antibody MGC119511 antibody OSF 2 antibody OSF-2 antibody OSF2 antibody Osteoblast specific factor 2 (fasciclin I like) antibody Osteoblast specific factor 2 antibody Osteoblast specific factor antibody Osteoblast-specific factor 2 antibody PDLPOSTN antibody Periodontal ligament specific periostin antibody Periostin antibody Periostin isoform thy2 antibody Periostin isoform thy4 antibody Periostin isoform thy6 antibody Periostin isoform thy8 antibody Periostin osteoblast specific factor antibody PN antibody POSTN antibody RP11 412K4.1 antibody |
| Molecular Weight(MW) | 93kDa(Observed band size: 85kDa) |
| Cellular Localization | Golgi apparatus, Secreted, extracellular space, extracellular matrix. |
WB
Western blot analysis of Periostin on different lysates with Rabbit anti-Periostin antibody at 1/5,000 dilution. Lane 1: Mouse skin tissue lysate, Lane 2: Mouse spleen tissue lysate, Lane 3: Mouse skeletal muscle tissue lysate, Lane 4: Mouse stomach tissue lysate, Low expression: Mouse spleen, Mouse skeletal muscle. Lysates/proteins at 30 µg/Lane. Exposure time: 43 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 1/5,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP , 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
IHC
Species: Mouse, Tissue: Skin, Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Species: Mouse, Tissue: Stomach, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IF-F
Species: Mouse, Tissue: Stomach, Sample: Frozen section, Antigen retrieval: Not required Wash buffer: 1× TBST, Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 1.5 hours at room temperature.| Application Notes | WB:1:5000 IHC:1:1000 IF-F:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
