WB
Western blot analysis of TFEB using anti-TFEB antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TFEB antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.WB
Western blot analysis of TFEB using anti-TFEB antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TFEB antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of TFEB using anti-TFEB antibody. TFEB was detected in a paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-TFEB Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of TFEB using anti-TFEB antibody. TFEB was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-TFEB Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of TFEB using anti-TFEB antibody. TFEB was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-TFEB Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of TFEB using anti-TFEB antibody. TFEB was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-TFEB Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI(Blue).FC
Flow Cytometry analysis of U937 cells using anti-TFEB antibody. Overlay histogram showing U937 cells stained with anti-TFEB antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TFEB Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.IF-P
IF analysis using anti- TFEB antibody. detected in paraffin-embedded section of human intestinal cancer tissue. The tissue section were stained using the Fluoro550-conjugated Anti-rabbit IgG Secondary Antibody (red)and counterstained with DAPI (blue).IF-P
IF analysis using anti- TFEB antibody. detected in paraffin-embedded section of human breast cancer tissue. The tissue section were stained using the Fluoro550-conjugated Anti-rabbit IgG Secondary Antibody (red)and counterstained with DAPI (blue).| Product Name | Rabbit polyclonal antibody to TFEB |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human TFEB recombinant protein (Position: A214-H326). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 IF-P:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TFEB |
|---|---|
| Gene Synonyms | TCFEB BHLHE35 ALPHATFEB |
| Gene Full Name | transcription factor EB |
| Gene Summary | Enables DNA-binding transcription factor activity; enzyme binding activity; and transcription cis-regulatory region binding activity. Involved in several processes, including antibacterial innate immune response; cellular response to amino acid starvation; and positive regulation of metabolic process. Located in cytosol and lysosomal membrane. Is active in nucleus. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | AlphaTFEB bHLHe35 TCFEB TFEB transcription factor EB |
| Molecular Weight(MW) | 53 kDa(Observed band size: 60-75 kDa) |
| Cellular Localization | Cytoplasm,Lysosome,Membrane,Nucleus |
WB
Western blot analysis of TFEB using anti-TFEB antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TFEB antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
WB
Western blot analysis of TFEB using anti-TFEB antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TFEB antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of TFEB using anti-TFEB antibody. TFEB was detected in a paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-TFEB Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of TFEB using anti-TFEB antibody. TFEB was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-TFEB Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of TFEB using anti-TFEB antibody. TFEB was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-TFEB Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of TFEB using anti-TFEB antibody. TFEB was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-TFEB Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI(Blue).
FC
Flow Cytometry analysis of U937 cells using anti-TFEB antibody. Overlay histogram showing U937 cells stained with anti-TFEB antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TFEB Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF-P
IF analysis using anti- TFEB antibody. detected in paraffin-embedded section of human intestinal cancer tissue. The tissue section were stained using the Fluoro550-conjugated Anti-rabbit IgG Secondary Antibody (red)and counterstained with DAPI (blue).
IF-P
IF analysis using anti- TFEB antibody. detected in paraffin-embedded section of human breast cancer tissue. The tissue section were stained using the Fluoro550-conjugated Anti-rabbit IgG Secondary Antibody (red)and counterstained with DAPI (blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 IF-P:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
