WB
Western blot analysis of nlz2 znf503 using anti-nlz2 znf503 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-nlz2 znf503 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
IF analysis of nlz2 znf503 using anti-nlz2 znf503 antibody. nlz2 znf503 was detected in a paraffin-embedded section of IF analysis of nlz2 using anti-nlz2 antibody. nlz2 was detected in paraffin-embedded section of five-day-old zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 4μg/mL rabbit anti-nlz2 Antibody overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. tissue. The tissue section was incubated with rabbit anti-nlz2 znf503 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody.| Product Name | Rabbit polyclonal antibody to Zebrafish nlz2 znf503 |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PWB |
| WB:1:500-1:2000 IF-P:1:50-1:400 |
|
| Species Reactivity | Zebrafish |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZNF503 |
|---|---|
| Gene Synonyms | Nlz2 ZPO2 NOLZ1 NOLZ-1 ZEPPO2 |
| Gene Full Name | zinc finger protein 503 |
| Gene Summary | Predicted to enable zinc ion binding activity. Involved in G1 to G0 transition involved in cell differentiation; negative regulation of cell population proliferation; and negative regulation of gene expression. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025] |
| Molecular Weight(MW) | 63 kDa |
| Cellular Localization | Nucleus |
WB
Western blot analysis of nlz2 znf503 using anti-nlz2 znf503 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-nlz2 znf503 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
IF analysis of nlz2 znf503 using anti-nlz2 znf503 antibody. nlz2 znf503 was detected in a paraffin-embedded section of IF analysis of nlz2 using anti-nlz2 antibody. nlz2 was detected in paraffin-embedded section of five-day-old zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 4μg/mL rabbit anti-nlz2 Antibody overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. tissue. The tissue section was incubated with rabbit anti-nlz2 znf503 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody.| Application Notes | WB:1:500-1:2000 IF-P:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
