WB
Western blot analysis of lysates from 293 cells, using Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit pAb. 293 cells were treated with EGF. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.IHC
Immunohistochemistry analysis of paraffin embedded Human placenta using Phospho HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Mouse spleen using Phospho HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Rat brain using Phospho-HDAC4 S246/HDAC5-S259/HDAC9-S220 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.| Product Name | Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. |
| Modification | P-S246+S259+S220 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:1000 IHC:1:150-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HDAC4,HDAC5,HDAC9 |
|---|---|
| Gene Synonyms | HD4 AHO3 BDMR HDACA HA6116 HDAC-4 HDAC-A NEDCHF NEDCHID |
| Gene Full Name | histone deacetylase 4,5,9 |
| Gene Summary | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008] |
| Alternative Names | HDAC4/HDAC5/HDAC9 Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 |
| Molecular Weight(MW) | 125kDa/140kDa |
| Cellular Localization | cytoplasm, cytosol, histone deacetylase complex, nuclear speck, nucleoplasm, nucleus. |
WB
Western blot analysis of lysates from 293 cells, using Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit pAb. 293 cells were treated with EGF. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.
IHC
Immunohistochemistry analysis of paraffin embedded Human placenta using Phospho HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse spleen using Phospho HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Rat brain using Phospho-HDAC4 S246/HDAC5-S259/HDAC9-S220 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.| Application Notes | WB:1:500-1:1000 IHC:1:150-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
