WB
Western blot analysis of VAChT in mouse brain,mouse eyes and rat brain lysates using VAChT Goat pAb.ICC/IF
Immunofluorescence analysis of VAChT(Green) in mouse brain tissue using VAChT Goat pAb, and DAPI(Blue).IHC
Immunohistochemistry of Paraffin-embedded mouse brain tissue sections using the antibody VAChT Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using sodium citrate buffer (pH 6.0).| Product Name | VAChT Goat pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human VAChT |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | IF-PIHCWB |
| WB:1:500-1:1000 IHC:1:100-1:500 IF-P:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC18A3 |
|---|---|
| Gene Synonyms | CMS21 VACHT |
| Gene Full Name | solute carrier family 18 member A3 |
| Gene Summary | This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | Vesicular Acetylcholine Transporter MGC12716 rVAT SLC18A3 Solute carrier family 18 (vesicular acetylcholine) member 3 Solute carrier family 18 (vesicular monoamine) member 3 Solute carrier family 18 member 3 VACht VAChT VACHT_HUMAN |
| Molecular Weight(MW) | 70 kDa(Observed MW: 70 kDa) |
| Cellular Localization | Cytoplasmic vesicle,Membrane,Synapse |
WB
Western blot analysis of VAChT in mouse brain,mouse eyes and rat brain lysates using VAChT Goat pAb.
ICC/IF
Immunofluorescence analysis of VAChT(Green) in mouse brain tissue using VAChT Goat pAb, and DAPI(Blue).
IHC
Immunohistochemistry of Paraffin-embedded mouse brain tissue sections using the antibody VAChT Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using sodium citrate buffer (pH 6.0).| Application Notes | WB:1:500-1:1000 IHC:1:100-1:500 IF-P:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS, 50% glycerol, 0.05% Proclin 300, 0.08%BSA. |
