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WB
Western blot analysis of VAChT in mouse brain,mouse eyes and rat brain lysates using VAChT Goat pAb.IF
Immunofluorescence analysis of VAChT(Green) in mouse brain tissue using VAChT Goat pAb, and DAPI(Blue)IHC
Immunohistochemistry of Paraffin-embedded mouse brain tissue sections using the antibody VAChT Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using sodium citrate buffer (pH 6.0).| Product Name | VAChT Goat pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human VAChT |
| Modification | Unmodified |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | IFIHCWB |
| WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Symbol | SLC18A3 |
|---|---|
| Gene Synonyms | CMS21 VACHT |
| Gene Full Name | solute carrier family 18 member A3 |
| Gene Summary | This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 70 kDa |
| Function | Vesicular Acetylcholine Transporter (VAChT), (~70kD protein), belongs to the family of vesicular monoamine transporters(VMATs), which include VMAT1 and VMAT2 and the C. elegans putative ACh transporter unc-17. Members of this family function to concentrate neurotransmitters into synaptic vesicles through exchange of protons for neurotransmitters. VAChT is a functional transporter for the neurotransmitter acetylcholine(ACh). ACh is synthesized in the cytoplasm by choline acetyl transferase (ChAT) and transported by VAChT into synaptic vesicles where it is stored until released. After release from presynaptic nerve terminals ACh is hydrolyzed by extracellular ACh-esterases(AChE) to choline and acetate. VAChT mRNA is expressed in all known major cholinergic neurons in the central and peripheral nervous system. VAChT is abundantly expressed in the CNS and is mainly localized in small synaptic vesicles in cholinergic nerve terminals. VAChT provides a specific marker for cholinergic neurons for the study of cholinergic transmission in experimental models, in Alzheimer’s disease and other nervous system disorders. |
| Cellular Localization | Cytoplasmic vesicle,Membrane,Synapse |
WB
Western blot analysis of VAChT in mouse brain,mouse eyes and rat brain lysates using VAChT Goat pAb.
IF
Immunofluorescence analysis of VAChT(Green) in mouse brain tissue using VAChT Goat pAb, and DAPI(Blue)
IHC
Immunohistochemistry of Paraffin-embedded mouse brain tissue sections using the antibody VAChT Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using sodium citrate buffer (pH 6.0).| Application Notes | WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS, 50% glycerol, 0.05% Proclin 300, 0.08%BSA |
