WB
Western blot analysis of YTHDF2 using anti-YTHDF2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-YTHDF2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody.The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of YTHDF2 using anti-YTHDF2 antibody.YTHDF2 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-YTHDF2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of YTHDF2 using anti-YTHDF2 antibody.YTHDF2 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-YTHDF2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of YTHDF2 using anti-YTHDF2 antibody.YTHDF2 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-YTHDF2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of YTHDF2 using anti-YTHDF2 antibody.YTHDF2 was detected in an immunocytochemical section of HepG2 cells. The section was incubated with rabbit anti-YTHDF2 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI.FC
Flow Cytometry analysis of Raw264.7 cells using anti-YTHDF2 antibody.Overlay histogram showing Raw264.7 cells stained with anti-YTHDF2 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-YTHDF2 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to YTHDF2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human YTHDF2, identical to the related mouse and rat sequences. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:10-1:50 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | YTHDF2 |
|---|---|
| Gene Synonyms | DF2 CAHL HGRG8 NY-REN-2 |
| Gene Full Name | YTH N6-methyladenosine RNA binding protein F2 |
| Gene Summary | This gene encodes a member of the YTH (YT521-B homology) superfamily containing YTH domain. The YTH domain is typical for the eukaryotes and is particularly abundant in plants. The YTH domain is usually located in the middle of the protein sequence and may function in binding to RNA. In addition to a YTH domain, this protein has a proline rich region which may be involved in signal transduction. An Alu-rich domain has been identified in one of the introns of this gene, which is thought to be associated with human longevity. In addition, reciprocal translocations between this gene and the Runx1 (AML1) gene on chromosome 21 has been observed in patients with acute myeloid leukemia. This gene was initially mapped to chromosome 14, which was later turned out to be a pseudogene. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Oct 2012] |
| Alternative Names | CLL associated antigen KW 14 HGRG8 NY REN 2 YTH domain family protein 2 YTH domain family member 2 YTHDF2 |
| Molecular Weight(MW) | 62 kDa(Observed band size: 65 kDa) |
| Cellular Localization | Cytoplasm,Nucleus |
WB
Western blot analysis of YTHDF2 using anti-YTHDF2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-YTHDF2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody.The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of YTHDF2 using anti-YTHDF2 antibody.YTHDF2 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-YTHDF2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of YTHDF2 using anti-YTHDF2 antibody.YTHDF2 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-YTHDF2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of YTHDF2 using anti-YTHDF2 antibody.YTHDF2 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-YTHDF2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of YTHDF2 using anti-YTHDF2 antibody.YTHDF2 was detected in an immunocytochemical section of HepG2 cells. The section was incubated with rabbit anti-YTHDF2 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI.
FC
Flow Cytometry analysis of Raw264.7 cells using anti-YTHDF2 antibody.Overlay histogram showing Raw264.7 cells stained with anti-YTHDF2 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-YTHDF2 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:10-1:50 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
