WB
Western blot analysis of P-Selectin / CD62P on different lysates with Rabbit anti-P-Selectin / CD62P antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate (negative) (20 µg/Lane), Lane 2: HEL cell lysate (20 µg/Lane), Lane 3: Mouse placenta tissue lysate (40 µg/Lane), Lane 4: Mouse lung tissue lysate (40 µg/Lane), Lane 5: Rat placenta tissue lysate (40 µg/Lane), Lane 6: Rat lung tissue lysate (40 µg/Lane), Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-P-Selectin / CD62P antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-P-Selectin / CD62P antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/337 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-P-Selectin / CD62P antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Species: Mouse, Site: spleen, Sample: Frozen section, Antibody concentration: 1/1,000, Antigen retrieval: Not required.FC
Flow cytometric analysis of mouse platelets labeling P-Selectin / CD62P. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IP
P-Selectin / CD62P was immunoprecipitated from 0.2 mg HEL cell lysate with Rabbit anti-P-Selectin / CD62P antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-P-Selectin / CD62P antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HEL cell lysate (input), Lane 2: Rabbit anti-P-Selectin / CD62P antibody IP in HEL cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-P-Selectin / CD62P antibody in HEL cell lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 1 minute.| Product Name | P-Selectin / CD62P Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Mouse P-Selectin aa 42-768. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-FIHCIPWB |
| WB:1:2000-1:5000 IHC:1:50-1:200 IF-F:1:1000 FC:1:1000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SELP |
|---|---|
| Gene Synonyms | CD62 GRMP PSEL CD62P GMP140 LECAM3 PADGEM |
| Gene Full Name | selectin P |
| Gene Summary | This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. [provided by RefSeq, Jul 2008] |
| Alternative Names | CD62P antibody GMRP antibody GRMP antibody SELP antibody P-selectin antibody CD62 antigen-like family member P antibody Granule membrane protein 140 antibody Leukocyte-endothelial cell adhesion molecule 3 antibody Platelet activation dependent granule-external membrane protein antibody GMP-140 antibody CD62P antibody GMRP antibody GRMP antibody SELP antibody P-selectin antibody CD62 antigen-like family member P antibody Granule membrane protein 140 antibody Leukocyte-endothelial cell adhesion molecule 3 antibody Platelet activation dependent granule-external membrane protein antibody GMP-140 antibody LECAM3 antibody PADGEM antibody |
| Molecular Weight(MW) | 83 kDa(Observed band size: 130-150 kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of P-Selectin / CD62P on different lysates with Rabbit anti-P-Selectin / CD62P antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate (negative) (20 µg/Lane), Lane 2: HEL cell lysate (20 µg/Lane), Lane 3: Mouse placenta tissue lysate (40 µg/Lane), Lane 4: Mouse lung tissue lysate (40 µg/Lane), Lane 5: Rat placenta tissue lysate (40 µg/Lane), Lane 6: Rat lung tissue lysate (40 µg/Lane), Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-P-Selectin / CD62P antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-P-Selectin / CD62P antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/337 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-P-Selectin / CD62P antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Species: Mouse, Site: spleen, Sample: Frozen section, Antibody concentration: 1/1,000, Antigen retrieval: Not required.
FC
Flow cytometric analysis of mouse platelets labeling P-Selectin / CD62P. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
P-Selectin / CD62P was immunoprecipitated from 0.2 mg HEL cell lysate with Rabbit anti-P-Selectin / CD62P antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-P-Selectin / CD62P antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HEL cell lysate (input), Lane 2: Rabbit anti-P-Selectin / CD62P antibody IP in HEL cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-P-Selectin / CD62P antibody in HEL cell lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 1 minute.| Application Notes | WB:1:2000-1:5000 IHC:1:50-1:200 IF-F:1:1000 FC:1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
