WB
Western blot analysis of alpha 1a Adrenergic Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody was used in 5% NFDM/TBST at room temperature for 2 hours. at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate; Lane 2: SH-SY5Y cell lysate; Lane 3: PC-3M cell lysate; Lane 4: Mouse lung tissue lysateGoat Anti-Rabbit IgG - HRP Secondary Antibody .ICC/IF
ICC staining of alpha 1a Adrenergic Receptor in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperatu(1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).re. Cells were probed with the primary antibody .ICC/IF
ICC staining of alpha 1a Adrenergic Receptor in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50)for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).IHC
Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-alpha 1a Adrenergic Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.FC
Flow cytometric analysis of alpha 1a Adrenergic Receptor was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control ( green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | alpha 1a Adrenergic Receptor Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human alpha 1a Adrenergic Receptor aa 200-250/466. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:100 IHC:1:50-1:200 FC:1:500-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ADRA1A |
|---|---|
| Gene Synonyms | ADRA1C ADRA1L1 ALPHA1AAR |
| Gene Full Name | adrenoceptor alpha 1A |
| Gene Summary | Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. [provided by RefSeq, Jul 2008] |
| Alternative Names | ADA1A_HUMAN antibody ADRA1A antibody Adra1c antibody ADRA1L1 antibody Adrenergic alpha1A receptor antibody Adrenergic alpha1C receptor antibody Adrenergic receptor alpha 1a antibody Adrenergic receptor alpha 1c antibody Adrenergic alpha 1A receptor variant 1 antibody Adrenergic alpha 1A receptor variant 11 antibody ADA1A_HUMAN antibody ADRA1A antibody Adra1c antibody ADRA1L1 antibody Adrenergic alpha1A receptor antibody Adrenergic alpha1C receptor antibody Adrenergic receptor alpha 1a antibody Adrenergic receptor alpha 1c antibody Adrenergic alpha 1A receptor variant 1 antibody Adrenergic alpha 1A receptor variant 11 antibody Adrenergic alpha 1A receptor variant 3 antibody Adrenergic alpha 1A receptor variant 5 antibody Adrenergic alpha 1A receptor variant 8 antibody Adrenoceptor alpha 1A antibody Alpha 1A adrenoreceptor antibody Alpha 1C adrenergic receptor antibody Alpha adrenergic receptor 1c antibody Alpha-1A adrenergic receptor antibody Alpha-1A adrenoceptor antibody Alpha-1A adrenoreceptor antibody Alpha-1C adrenergic receptor antibody Alpha-adrenergic receptor 1c antibody Alpha1A adrenergic receptor antibody ALPHA1AAR antibody G protein coupled receptor antibody |
| Molecular Weight(MW) | 51 kDa |
| Cellular Localization | Cytoplasm, Cell membrane, Nucleus membrane, caveola. |
WB
Western blot analysis of alpha 1a Adrenergic Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody was used in 5% NFDM/TBST at room temperature for 2 hours. at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate; Lane 2: SH-SY5Y cell lysate; Lane 3: PC-3M cell lysate; Lane 4: Mouse lung tissue lysateGoat Anti-Rabbit IgG - HRP Secondary Antibody .
ICC/IF
ICC staining of alpha 1a Adrenergic Receptor in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperatu(1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).re. Cells were probed with the primary antibody .
ICC/IF
ICC staining of alpha 1a Adrenergic Receptor in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50)for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
IHC
Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-alpha 1a Adrenergic Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
FC
Flow cytometric analysis of alpha 1a Adrenergic Receptor was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control ( green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:100 IHC:1:50-1:200 FC:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
