WB
Western blot analysis of CYP11A1 on different lysates with Rabbit anti-CYP11A1 antibody at 1/2,000 dilution.Exposure time: 1 minute; 4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | CYP11A1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human CYP11A1 aa 1-350. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CYP11A1 |
|---|---|
| Gene Synonyms | CYP11A CYPXIA1 P450SCC |
| Gene Full Name | cytochrome P450 family 11 subfamily A member 1 |
| Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008] |
| Alternative Names | Cholesterol 20 22 desmolase antibody Cholesterol desmolase antibody Cholesterol monooxygenase (side chain cleaving) antibody Cholesterol side chain cleavage enzyme antibody Cholesterol side chain cleavage enzyme mitochondrial antibody Cholesterol side-chain cleavage enzyme antibody CP11A_HUMAN antibody CYP11A antibody CYP11A1 antibody CYPXIA1 antibody Cholesterol 20 22 desmolase antibody Cholesterol desmolase antibody Cholesterol monooxygenase (side chain cleaving) antibody Cholesterol side chain cleavage enzyme antibody Cholesterol side chain cleavage enzyme mitochondrial antibody Cholesterol side-chain cleavage enzyme antibody CP11A_HUMAN antibody CYP11A antibody CYP11A1 antibody CYPXIA1 antibody Cytochrome P450 11A1 antibody Cytochrome P450 11A1 mitochondrial antibody Cytochrome P450 family 11 subfamily A polypeptide 1 antibody Cytochrome P450 subfamily XIA antibody Cytochrome P450(scc) antibody Cytochrome P450C11A1 antibody mitochondrial antibody P450SCC antibody Steroid 20 22 lyase antibody |
| Molecular Weight(MW) | 60 kDa(Observed band size: 45 kDa) |
| Cellular Localization | Mitochondrion inner membrane. |
WB
Western blot analysis of CYP11A1 on different lysates with Rabbit anti-CYP11A1 antibody at 1/2,000 dilution.Exposure time: 1 minute; 4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
