WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-HuD/HuC antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human brain was stained with anti-HuD/HuC Rabbit antibody| Product Name | HuD/HuC Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000-1:10000 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ELAVL3 |
|---|---|
| Gene Synonyms | HUC HUCL PLE21 |
| Gene Full Name | ELAV like RNA binding protein 3 |
| Gene Summary | A member of the ELAVL protein family, ELAV-like 3 is a neural-specific RNA-binding protein which contains three RNP-type RNA recognition motifs. The observation that ELAVL3 is one of several Hu antigens (neuronal-specific RNA-binding proteins) recognized by the anti-Hu serum antibody present in sera from patients with paraneoplastic encephalomyelitis and sensory neuronopathy (PEM/PSN) suggests it has a role in neurogenesis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | Hu-antigen D HuD Paraneoplastic encephalomyelitis antigen HuD ELAV-like protein 3 Hu-antigen C HuC Paraneoplastic cerebellar degeneration-associated antigen Paraneoplastic limbic encephalitis antigen 21 |
| Molecular Weight(MW) | 40,42 kDa(Observed band size: 39 kDa) |
| Cellular Localization | Cytoplasm . Perikaryon . Cell projection , dendrite . Cell projection , axon . Cell projection , growth cone . Co-localizes with ribosomal RNA in polysomes. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-HuD/HuC antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human brain was stained with anti-HuD/HuC Rabbit antibody| Application Notes | WB:1:2000-1:10000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
