WB
Western blot analysis of Mov10 on different lysates with Rabbit anti-Mov10 antibody at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate (20 µg/Lane),
Lane 2: HeLa cell lysate (20 µg/Lane),
Lane 3: HepG2 cell lysate (20 µg/Lane),
Lane 4: NIH/3T3 cell lysate (20 µg/Lane),
Lane 5: RAW264.7 cell lysate (20 µg/Lane),
Lane 6: C6 cell lysate (20 µg/Lane),
Lane 6: Rat liver tissue lysate (40 µg/Lane),
Lane 6: Rat kidney tissue lysate (40 µg/Lane),
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.