WB
Western blot analysis of TAZ / WWTR1 on different lysates with Rabbit anti-TAZ / WWTR1 antibody at 1/5,000 dilution.Lysates/proteins at 20 µg/Lane.Exposure time: 3 minutes;4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-TAZ / WWTR1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-TAZ / WWTR1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TAZ / WWTR1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-TAZ / WWTR1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
TAZ / WWTR1 was immunoprecipitated from 0.2 mg PANC-1 cell lysate with Rabbit anti-TAZ / WWTR1 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-TAZ / WWTR1 antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: PANC-1 cell lysate (input) Lane 2: Rabbit anti-TAZ / WWTR1 antibody IP in PANC-1 cell lysate Lane 3: Rabbit IgG instead of Rabbit anti-TAZ / WWTR1 antibody in PANC-1 cell lysate Blocking/Dilution buffer: primary antibody dilution Exposure time: 3 minutes;IP
TAZ / WWTR1 was immunoprecipitated from 0.2 mg C2C12 cell lysate with Rabbit anti-TAZ / WWTR1 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-TAZ / WWTR1 antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: C2C12 cell lysate (input) Lane 2: Rabbit anti-TAZ / WWTR1 antibody IP in C2C12 cell lysate Lane 3: Rabbit IgG instead of Rabbit anti-TAZ / WWTR1 antibody in C2C12 cell lysate Blocking/Dilution buffer: primary antibody dilution Exposure time: 20 seconds;| Product Name | TAZ / WWTR1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human TAZ / WWTR1 aa 1-400. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:5000 IHC:1:200-1:1000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | WWTR1 |
|---|---|
| Gene Synonyms | TAZ |
| Gene Full Name | WW domain containing transcription regulator 1 |
| Gene Summary | Enables transcription coactivator activity. Involved in several processes, including intracellular signal transduction; negative regulation of canonical Wnt signaling pathway; and positive regulation of cell differentiation. Located in cytosol and nuclear body. Is active in nucleus. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | DKFZP586I1419 antibody FLJ27004 antibody FLJ45718 antibody OTTHUMP00000215994 antibody OTTHUMP00000215995 antibody OTTHUMP00000215996 antibody OTTHUMP00000216001 antibody TAZ antibody Transcriptional co activator with PDZ binding motif antibody Transcriptional coactivator with PDZ binding motif antibody DKFZP586I1419 antibody FLJ27004 antibody FLJ45718 antibody OTTHUMP00000215994 antibody OTTHUMP00000215995 antibody OTTHUMP00000215996 antibody OTTHUMP00000216001 antibody TAZ antibody Transcriptional co activator with PDZ binding motif antibody Transcriptional coactivator with PDZ binding motif antibody Transcriptional coactivator with PDZ-binding motif antibody WW domain containing transcription regulator 1 antibody WW domain containing transcription regulator protein 1 antibody WW domain-containing transcription regulator protein 1 antibody WWTR 1 antibody WWTR1 antibody WWTR1_HUMAN antibody |
| Molecular Weight(MW) | 44 kDa(Observed band size: 50 kDa) |
| Cellular Localization | Nucleus, Cytoplasm, Cell membrane, Cell junction, tight junction. |
WB
Western blot analysis of TAZ / WWTR1 on different lysates with Rabbit anti-TAZ / WWTR1 antibody at 1/5,000 dilution.Lysates/proteins at 20 µg/Lane.Exposure time: 3 minutes;4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-TAZ / WWTR1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-TAZ / WWTR1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TAZ / WWTR1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-TAZ / WWTR1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
TAZ / WWTR1 was immunoprecipitated from 0.2 mg PANC-1 cell lysate with Rabbit anti-TAZ / WWTR1 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-TAZ / WWTR1 antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: PANC-1 cell lysate (input) Lane 2: Rabbit anti-TAZ / WWTR1 antibody IP in PANC-1 cell lysate Lane 3: Rabbit IgG instead of Rabbit anti-TAZ / WWTR1 antibody in PANC-1 cell lysate Blocking/Dilution buffer: primary antibody dilution Exposure time: 3 minutes;
IP
TAZ / WWTR1 was immunoprecipitated from 0.2 mg C2C12 cell lysate with Rabbit anti-TAZ / WWTR1 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-TAZ / WWTR1 antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: C2C12 cell lysate (input) Lane 2: Rabbit anti-TAZ / WWTR1 antibody IP in C2C12 cell lysate Lane 3: Rabbit IgG instead of Rabbit anti-TAZ / WWTR1 antibody in C2C12 cell lysate Blocking/Dilution buffer: primary antibody dilution Exposure time: 20 seconds;| Application Notes | WB:1:5000 IHC:1:200-1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
