WB
Western blot analysis of MTBR-TAU 243 lsoform 2N4R (236-250) on different lysates with Rabbit anti-MTBR-TAU 243 lsoform 2N4R (236-250) antibody at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate, Lane 2: Mouse spleen tissue lysate (negative), Lane 3: Mouse cerebellum tissue lysate, Lane 4: Mouse testis tissue lysate (negative), Lane 5: Rat brain tissue lysate, Lane 6: Rat spleen tissue lysate (negative), Lane 7: Rat cerebellum tissue lysate, Lane 8: Rat testis tissue lysate (negative), Lane 9: Pig brain tissue lysate, Lane 10: Pig spleen tissue lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Species: Mouse, Tissue: Alzheimer's brain, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/30,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Species: Mouse, Tissue: Brain, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/30,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Species: Mouse, Tissue: Cerebellum, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/30,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Species: Mouse, Tissue: Spleen, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/10,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature. Negative control: spleen.IP
50 ng of full-length TAU isoform 2N4R protein (1-441 aa) and 200 ng of truncated TAU isoform 2N4R protein (1-256 aa) were incubated with 0.2 mg of negative tissue lysate from mouse spleen, along with Rabbit anti-MTBR-TAU 243 lsoform 2N4R (236-250) antibody at 2 µg/10 µl of beads. Western blot was performed from the immunoprecipitate using Rabbit anti-MTBR-TAU 243 lsoform 2N4R (236-250) antibody at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: TAU isoform 2N4R (full-length protein, amino acids 1–441) and TAU isoform 2N4R (truncated protein, amino acids 1–256) were spiked into negative tissue lysate from mouse spleen (input), Lane 2: Rabbit anti-MTBR-TAU 243 lsoform 2N4R (236-250) antibody IP in TAU isoform 2N4R (full-length protein, amino acids 1–441) and TAU isoform 2N4R (truncated protein, amino acids 1–256) were spiked into negative tissue lysate from mouse spleen, Lane 3: Rabbit IgG instead of Rabbit anti-MTBR-TAU 243 lsoform 2N4R (236-250) antibody in TAU isoform 2N4R (full-length protein, amino acids 1–441) and TAU isoform 2N4R (truncated protein, amino acids 1–256) were spiked into negative tissue lysate from mouse spleen, Exposure time: 16 seconds, Blocking: 5% NFDM/TBST, 1 hour at room temperature.FC
Flow cytometric analysis of EL4 (left, negative) and Neuro-2a (right, positive) cells labeling MTBR-TAU 243 lsoform 2N4R (236-250). Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | MTBR-TAU 243 lsoform 2N4R (236-250) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human MAPT aa 236-250. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCIPWB |
| WB:1:5000 IHC:1:30000 IP:1-2μg/sample FC:1:1000 |
|
| Species Reactivity | HumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MAPT |
|---|---|
| Gene Synonyms | TAU FTD1 MSTD PPND DDPAC MAPTL MTBT1 MTBT2 tau-40 FTDP-17 PPP1R103 Tau-PHF6 |
| Gene Full Name | microtubule associated protein tau |
| Gene Summary | This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008] |
| Alternative Names | AI413597 antibody AW045860 antibody DDPAC antibody FLJ31424 antibody FTDP 17 antibody G protein beta1/gamma2 subunit interacting factor 1 antibody MAPT antibody MAPTL antibody MGC134287 antibody MGC138549 antibody AI413597 antibody AW045860 antibody DDPAC antibody FLJ31424 antibody FTDP 17 antibody G protein beta1/gamma2 subunit interacting factor 1 antibody MAPT antibody MAPTL antibody MGC134287 antibody MGC138549 antibody MGC156663 antibody Microtubule associated protein tau antibody Microtubule associated protein tau isoform 4 antibody Microtubule-associated protein tau antibody MSTD antibody Mtapt antibody MTBT1 antibody MTBT2 antibody Neurofibrillary tangle protein antibody Paired helical filament tau antibody Paired helical filament-tau antibody PHF tau antibody PHF-tau antibody PPND antibody PPP1R103 antibody Protein phosphatase 1 regulatory subunit 103 antibody pTau antibody RNPTAU antibody TAU antibody Tauopathy and respiratory failure included antibody |
| Molecular Weight(MW) | Observed band size: 50-80 kDa |
| Cellular Localization | Cytoplasm, cytosol, Cell membrane, cytoskeleton, Cell projection, axon, dendrite, Secreted. |
WB
Western blot analysis of MTBR-TAU 243 lsoform 2N4R (236-250) on different lysates with Rabbit anti-MTBR-TAU 243 lsoform 2N4R (236-250) antibody at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate, Lane 2: Mouse spleen tissue lysate (negative), Lane 3: Mouse cerebellum tissue lysate, Lane 4: Mouse testis tissue lysate (negative), Lane 5: Rat brain tissue lysate, Lane 6: Rat spleen tissue lysate (negative), Lane 7: Rat cerebellum tissue lysate, Lane 8: Rat testis tissue lysate (negative), Lane 9: Pig brain tissue lysate, Lane 10: Pig spleen tissue lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Species: Mouse, Tissue: Alzheimer's brain, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/30,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Species: Mouse, Tissue: Brain, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/30,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Species: Mouse, Tissue: Cerebellum, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/30,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Species: Mouse, Tissue: Spleen, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/10,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature. Negative control: spleen.
IP
50 ng of full-length TAU isoform 2N4R protein (1-441 aa) and 200 ng of truncated TAU isoform 2N4R protein (1-256 aa) were incubated with 0.2 mg of negative tissue lysate from mouse spleen, along with Rabbit anti-MTBR-TAU 243 lsoform 2N4R (236-250) antibody at 2 µg/10 µl of beads. Western blot was performed from the immunoprecipitate using Rabbit anti-MTBR-TAU 243 lsoform 2N4R (236-250) antibody at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: TAU isoform 2N4R (full-length protein, amino acids 1–441) and TAU isoform 2N4R (truncated protein, amino acids 1–256) were spiked into negative tissue lysate from mouse spleen (input), Lane 2: Rabbit anti-MTBR-TAU 243 lsoform 2N4R (236-250) antibody IP in TAU isoform 2N4R (full-length protein, amino acids 1–441) and TAU isoform 2N4R (truncated protein, amino acids 1–256) were spiked into negative tissue lysate from mouse spleen, Lane 3: Rabbit IgG instead of Rabbit anti-MTBR-TAU 243 lsoform 2N4R (236-250) antibody in TAU isoform 2N4R (full-length protein, amino acids 1–441) and TAU isoform 2N4R (truncated protein, amino acids 1–256) were spiked into negative tissue lysate from mouse spleen, Exposure time: 16 seconds, Blocking: 5% NFDM/TBST, 1 hour at room temperature.
FC
Flow cytometric analysis of EL4 (left, negative) and Neuro-2a (right, positive) cells labeling MTBR-TAU 243 lsoform 2N4R (236-250). Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:5000 IHC:1:30000 IP:1-2μg/sample FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
