WB
Western blot analysis of Lipoamide Dehydrogenase on different lysates with Rabbit anti-Lipoamide Dehydrogenase antibody at 1/1,000 dilution.Exposure time: 1 minute; 4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Lipoamide Dehydrogenase antibody at 1/5,000 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Lipoamide Dehydrogenase antibody at 1/5,000 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling Lipoamide Dehydrogenase with Rabbit anti-Lipoamide Dehydrogenase antibody at 1/200 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Lipoamide Dehydrogenase antibody at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L(488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.| Product Name | Lipoamide Dehydrogenase Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human Lipoamide Dehydrogenase 21-509 / 509. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000 IHC:1:5000 ICC/IF:1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | DLD |
|---|---|
| Gene Synonyms | E3 LAD DLDD DLDH GCSL PHE3 OGDC-E3 |
| Gene Full Name | dihydrolipoamide dehydrogenase |
| Gene Summary | This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| Alternative Names | Dehydrogenase complex E3 component antibody Diaphorase antibody Dihydrolipoamide dehydrogenase antibody Dihydrolipoyl dehydrogenase antibody Dihydrolipoyl dehydrogenase mitochondrial antibody dld antibody DLDD antibody DLDH antibody DLDH_HUMAN antibody E3 antibody Dehydrogenase complex E3 component antibody Diaphorase antibody Dihydrolipoamide dehydrogenase antibody Dihydrolipoyl dehydrogenase antibody Dihydrolipoyl dehydrogenase mitochondrial antibody dld antibody DLDD antibody DLDH antibody DLDH_HUMAN antibody E3 antibody E3 branched chain aplha-keto acid antibody E3 component of pyruvate dehydrogenase antibody E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex antibody GCSL antibody Glycine cleavage system L protein antibody Glycine cleavage system protein L antibody LAD antibody lipoamide dehydrogenase antibody Lipoamide reductase antibody Lipoyl dehydrogenase antibody mitochondrial antibody OTTHUMP00000206744 antibody OTTHUMP00000206746 antibody OTTHUMP00000206748 antibody OTTHUMP00000206749 antibody PHE 3 antibody PHE3 antibody Pyruvate dehydrogenase component E3 antibody |
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Mitochondrion matrix, Nucleus, Cell projection, cilium, flagellum, Cytoplasmic vesicle, secretory vesicle, acrosome. |
WB
Western blot analysis of Lipoamide Dehydrogenase on different lysates with Rabbit anti-Lipoamide Dehydrogenase antibody at 1/1,000 dilution.Exposure time: 1 minute; 4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Lipoamide Dehydrogenase antibody at 1/5,000 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Lipoamide Dehydrogenase antibody at 1/5,000 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling Lipoamide Dehydrogenase with Rabbit anti-Lipoamide Dehydrogenase antibody at 1/200 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Lipoamide Dehydrogenase antibody at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L(488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.| Application Notes | WB:1:1000 IHC:1:5000 ICC/IF:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
