WB
Western Blot: Positive WB detected in: HepG2 whole cell lysate(30µg), Hela whole cell lysate(30µg), SY5Y whole cell lysate(30µg). All lanes: GPR75 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/20000 dilution.Exposure time: 120s.ICC/IF
Immunofluorescence staining of A549 cell with GPR75 Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).ICC/IF
Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-GPR75 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Probable G-protein coupled receptor 75 protein (372-540AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:20-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | GPR75 |
|---|---|
| Gene Synonyms | GPRchr2 WI31133 |
| Gene Full Name | G protein-coupled receptor 75 |
| Gene Summary | GPR75 is a member of the G protein-coupled receptor family. GPRs are cell surface receptors that activate guanine-nucleotide binding proteins upon the binding of a ligand.[supplied by OMIM, Jul 2002] |
| Alternative Names | G protein coupled receptor 75 antibody GPR chr2 antibody Gpr75 antibody GPR75_HUMAN antibody GPRchr2 antibody OTTHUMP00000159608 antibody Probable G protein coupled receptor 75 antibody Probable G-protein coupled receptor 75 antibody WI 31133 antibody WI31133 antibody |
| Molecular Weight(MW) | 60 kDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
WB
Western Blot: Positive WB detected in: HepG2 whole cell lysate(30µg), Hela whole cell lysate(30µg), SY5Y whole cell lysate(30µg). All lanes: GPR75 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/20000 dilution.Exposure time: 120s.
ICC/IF
Immunofluorescence staining of A549 cell with GPR75 Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
ICC/IF
Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:2000 ICC/IF:1:20-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
