IF-F
Application: IHC-Fr. Species: Mouse. Site: Paraventricular nucleus. Sample: Frozen section. Antibody concentration: 1/500. Antigen retrieval: Not required.IF-P
Application: IF-tissue. Species: Mouse. Site: Paraventricular nucleus. Sample: Paraffin-embedded section. Antibody concentration: 1/500.IF-P
Application: IF-tissue. Species: Mouse. Site: Pituitary. Sample: Paraffin-embedded section. Antibody concentration: 1/500.IF-P
Application: IF-tissue. Species: Rat. Site: Pituitary. Sample: Paraffin-embedded section. Antibody concentration: 1/500.IHC
Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Rat anti-Oxytocin-neurophysin 1 / OXT antibody at 1/5,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rat anti-Oxytocin-neurophysin 1 / OXT antibody at 1/5,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Oxytocin-neurophysin 1 / OXT Recombinant Antibody- Rat IgG1 (Chimeric) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human OXT aa 1-125. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Tested Applications | IF-FIF-PIHC |
| IF-F:1:500 IHC:1:5000-1:20000 IF-P:1:500-1:2000 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Oxt |
|---|---|
| Gene Synonyms | OT Oxy |
| Gene Full Name | oxytocin |
| Gene Summary | This gene encodes a preproprotein that is processed to produce oxytocin and neurophysin 1. Oxytocin is a posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin 1. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. The precursor seems to be activated while it is being transported along the axon to the posterior pituitary. This hormone contracts smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation, the stress response and complex sexual and maternal behavior, as well as in the regulation of water excretion, salt appetite, blood pressure and cardiovascular functions. Deletion of this gene in mouse reduces bone formation resulting in osteoporosis. [provided by RefSeq, Dec 2013] |
| Alternative Names | MGC126890 antibody MGC126892 antibody Neurophysin 1 antibody Neurophysin I antibody Ocytocin antibody OT antibody OT NPI antibody OXT antibody Oxytocin antibody Oxytocin Neurophysin preproprotein antibody MGC126890 antibody MGC126892 antibody Neurophysin 1 antibody Neurophysin I antibody Ocytocin antibody OT antibody OT NPI antibody OXT antibody Oxytocin antibody Oxytocin Neurophysin preproprotein antibody Oxytocin prepro (neurophysin I) antibody Oxytocin prepropeptide antibody Oxytocin-neurophysin 1 antibody |
| Molecular Weight(MW) | 13 kDa |
| Cellular Localization | Secreted. |
IF-F
Application: IHC-Fr. Species: Mouse. Site: Paraventricular nucleus. Sample: Frozen section. Antibody concentration: 1/500. Antigen retrieval: Not required.
IF-P
Application: IF-tissue. Species: Mouse. Site: Paraventricular nucleus. Sample: Paraffin-embedded section. Antibody concentration: 1/500.
IF-P
Application: IF-tissue. Species: Mouse. Site: Pituitary. Sample: Paraffin-embedded section. Antibody concentration: 1/500.
IF-P
Application: IF-tissue. Species: Rat. Site: Pituitary. Sample: Paraffin-embedded section. Antibody concentration: 1/500.
IHC
Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Rat anti-Oxytocin-neurophysin 1 / OXT antibody at 1/5,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rat anti-Oxytocin-neurophysin 1 / OXT antibody at 1/5,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | IF-F:1:500 IHC:1:5000-1:20000 IF-P:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.. |
