WB
Western blot analysis of Oxytocin-neurophysin 1 / OXT on different lysates with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody at 1/1,000 dilution. Lane 1: Mouse pituitary tissue lysate, Lane 2: Rat pituitary tissue lysate, Lysates/proteins at 40 µg/Lane. Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Immunofluorescence analysis of frozen rat supraoptic nucleus tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).IF-P
Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Oxytocin-neurophysin 1 / OXT Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human OXT aa 1-125. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIF-PIHCWB |
| WB:1:1000 IHC:1:2000-1:5000 IF-F:1:500 IF-P:1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Protein A |
| Gene Symbol | OXT |
|---|---|
| Gene Synonyms | OT OT-NPI OXT-NPI |
| Gene Full Name | oxytocin/neurophysin I prepropeptide |
| Gene Summary | This gene encodes a precursor protein that is processed to produce oxytocin and neurophysin I. Oxytocin is a posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. The precursor seems to be activated while it is being transported along the axon to the posterior pituitary. This hormone contracts smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. [provided by RefSeq, Dec 2013] |
| Alternative Names | MGC126890 antibody MGC126892 antibody Neurophysin 1 antibody Neurophysin I antibody Ocytocin antibody OT antibody OT NPI antibody OXT antibody Oxytocin antibody Oxytocin Neurophysin preproprotein antibody MGC126890 antibody MGC126892 antibody Neurophysin 1 antibody Neurophysin I antibody Ocytocin antibody OT antibody OT NPI antibody OXT antibody Oxytocin antibody Oxytocin Neurophysin preproprotein antibody Oxytocin prepro (neurophysin I) antibody Oxytocin prepropeptide antibody Oxytocin-neurophysin 1 antibody |
| Molecular Weight(MW) | 14kDa |
| Cellular Localization | Secreted. |
WB
Western blot analysis of Oxytocin-neurophysin 1 / OXT on different lysates with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody at 1/1,000 dilution. Lane 1: Mouse pituitary tissue lysate, Lane 2: Rat pituitary tissue lysate, Lysates/proteins at 40 µg/Lane. Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Immunofluorescence analysis of frozen rat supraoptic nucleus tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
IF-P
Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:2000-1:5000 IF-F:1:500 IF-P:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
