WB
Western blot analysis of anti- PI2/SERPINB1 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Use rabbit anti- PI2/SERPINB1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.ICC/IF
ICC/IF analysis of SERPINB1 using anti-SERPINB1 antibody.SERPINB1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-SERPINB1 Antibody at a dilution of 1:100.Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI(Blue).FC
Flow Cytometry analysis of SiHa cells using anti-SERPINB1 antibody. Overlay histogram showing SiHa cells stained with anti-SERPINB1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SERPINB1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.FC
Flow Cytometry analysis of THP-1 cells using anti-SERPINB1 antibody. Overlay histogram showing THP-1 cells stained with anti-SERPINB1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SERPINB1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to SERPINB1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human PI2/SERPINB1 recombinant protein (Position: A52-R275). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SERPINB1 |
|---|---|
| Gene Synonyms | EI LEI PI2 MNEI PI-2 HEL57 M/NEI ELANH2 HEL-S-27 |
| Gene Full Name | serpin family B member 1 |
| Gene Summary | The protein encoded by this gene is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
| Alternative Names | ELANH2 MNEI PI2 SERPINB1 Serpin B1 Peptidase inhibitor 2 PI-2 Monocyte/neutrophil elastase inhibitor EI M/NEI LEI Leukocyte elastase inhibitor |
| Molecular Weight(MW) | 43 kDa |
| Cellular Localization | Cytoplasm,Endosome,Lysosome,Secreted |
WB
Western blot analysis of anti- PI2/SERPINB1 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Use rabbit anti- PI2/SERPINB1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.
ICC/IF
ICC/IF analysis of SERPINB1 using anti-SERPINB1 antibody.SERPINB1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-SERPINB1 Antibody at a dilution of 1:100.Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI(Blue).
FC
Flow Cytometry analysis of SiHa cells using anti-SERPINB1 antibody. Overlay histogram showing SiHa cells stained with anti-SERPINB1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SERPINB1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
FC
Flow Cytometry analysis of THP-1 cells using anti-SERPINB1 antibody. Overlay histogram showing THP-1 cells stained with anti-SERPINB1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SERPINB1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
