WB
Western blot analysis of TRAM1L1 using anti-TRAM1L1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TRAM1L1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of TRAM1L1 using anti-TRAM1L1 antibody. TRAM1L1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-TRAM1L1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of TRAM1L1 using anti-TRAM1L1 antibody.TRAM1L1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-TRAM1L1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of TRAM1L1 using anti-TRAM1L1 antibody. TRAM1L1 was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-TRAM1L1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis using anti- TRAM1L1 antibody. detected in paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section were stained using the Fluoro550-conjugated Anti-rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue).FC
Flow Cytometry analysis of HEL cells using anti-TRAM1L1 antibody. Overlay histogram showing HEL cells stained with anti-TRAM1L1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAM1L1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to TRAM1L1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human TRAM1L1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRAM1L1 |
|---|---|
| Gene Full Name | translocation associated membrane protein 1 like 1 |
| Gene Summary | Predicted to be involved in protein insertion into ER membrane. Predicted to be located in endoplasmic reticulum and membrane. Predicted to be active in endoplasmic reticulum membrane. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | TRAM1L1 Translocating chain-associated membrane protein 1-like 1 |
| Molecular Weight(MW) | 42 kDa(Observed band size: 52 kDa) |
| Cellular Localization | Endoplasmic reticulum,Membrane |
WB
Western blot analysis of TRAM1L1 using anti-TRAM1L1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TRAM1L1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of TRAM1L1 using anti-TRAM1L1 antibody. TRAM1L1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-TRAM1L1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of TRAM1L1 using anti-TRAM1L1 antibody.TRAM1L1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-TRAM1L1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of TRAM1L1 using anti-TRAM1L1 antibody. TRAM1L1 was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-TRAM1L1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis using anti- TRAM1L1 antibody. detected in paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section were stained using the Fluoro550-conjugated Anti-rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue).
FC
Flow Cytometry analysis of HEL cells using anti-TRAM1L1 antibody. Overlay histogram showing HEL cells stained with anti-TRAM1L1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAM1L1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
