Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD40 ligand antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
Alternative Names
CD40LG CD40L TNFSF5 TRAP CD40 ligand CD40-L T-cell antigen Gp39 TNF-related activation protein TRAP Tumor necrosis factor ligand superfamily member 5 CD antigen CD154 [Cleaved into: CD40 ligand membrane form CD40 ligand soluble form]
Molecular Weight(MW)
29 kDa(Observed band size: 38 kDa)
Cellular Localization
Cell membrane ; Single-pass type II membrane protein . Cell surface .; [CD40 ligand , soluble form]: Secreted . Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa , actin polymerization , and a matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
Application
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD40 ligand antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.