WB
Western blot analysis of GFI1 using anti-GFI1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GFI1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of GFI1 using anti-GFI1 antibody. GFI1 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GFI1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.FC
Flow Cytometry analysis of THP-1 cells using anti-GFI1 antibody. Overlay histogram showing THP-1 cells stained with anti-GFI1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GFI1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to GFI1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human GFI1 recombinant protein (Position: M1-K422). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GFI1 |
|---|---|
| Gene Synonyms | SCN2 GFI-1 GFI1A ZNF163 |
| Gene Full Name | growth factor independent 1 transcriptional repressor |
| Gene Summary | This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | GFI 1 GFI1 Zinc finger protein 163 Zinc finger protein Gfi 1 ZNF163 |
| Molecular Weight(MW) | 45 kDa(Observed band size: 55 kDa) |
| Cellular Localization | Nucleus |
WB
Western blot analysis of GFI1 using anti-GFI1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GFI1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of GFI1 using anti-GFI1 antibody. GFI1 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GFI1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
FC
Flow Cytometry analysis of THP-1 cells using anti-GFI1 antibody. Overlay histogram showing THP-1 cells stained with anti-GFI1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GFI1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
