WB
Western blot analysis of SFXN1 using anti-SFXN1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SFXN1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate .IHC
IHC analysis of SFXN1 using anti-SFXN1 antibody. SFXN1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-SFXN1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of SFXN1 using anti-SFXN1 antibody. SFXN1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-SFXN1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).IP
IP analysis of SFXN1 using anti-SFXN1 antibody in HepG2 whole cell lysate. Western blot analysis of SFXN1 using anti- SFXN1 antibody. Lane 1: HepG2 whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- SFXN1 antibody in HepG2 whole cell lysate, Lane 3: anti- SFXN1 antibody (2μg) + HepG2 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- SFXN1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.FC
Flow Cytometry analysis of 293T cells using anti-SFXN1 antibody. Overlay histogram showing 293T cells stained with anti-SFXN1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFXN1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to SFXN1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human SFXN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IP:1:250-1:300 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SFXN1 |
|---|---|
| Gene Synonyms | TCC SLC56A1 |
| Gene Full Name | sideroflexin 1 |
| Gene Summary | Enables L-alanine transmembrane transporter activity and L-serine transmembrane transporter activity. Involved in L-alanine transport; L-serine transport; and serine import into mitochondrion. Located in mitochondrial inner membrane. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | sideroflexin 1 Sideroflexin-1 TCC Tricarboxylate carrier protein |
| Molecular Weight(MW) | 36 kDa(Observed band size: 35 kDa) |
| Cellular Localization | Membrane,Mitochondrion,Mitochondrion inner membrane |
WB
Western blot analysis of SFXN1 using anti-SFXN1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SFXN1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate .
IHC
IHC analysis of SFXN1 using anti-SFXN1 antibody. SFXN1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-SFXN1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of SFXN1 using anti-SFXN1 antibody. SFXN1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-SFXN1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IP
IP analysis of SFXN1 using anti-SFXN1 antibody in HepG2 whole cell lysate. Western blot analysis of SFXN1 using anti- SFXN1 antibody. Lane 1: HepG2 whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- SFXN1 antibody in HepG2 whole cell lysate, Lane 3: anti- SFXN1 antibody (2μg) + HepG2 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- SFXN1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
FC
Flow Cytometry analysis of 293T cells using anti-SFXN1 antibody. Overlay histogram showing 293T cells stained with anti-SFXN1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFXN1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IP:1:250-1:300 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
