WB
Western blot analysis of ABP1/AOC1 using anti-ABP1/AOC1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABP1/AOC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of ABP1/AOC1 using anti-ABP1/AOC1 antibody. ABP1/AOC1 was detected in a paraffin-embedded section of human Colonic adenoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ABP1/AOC1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of ABP1/AOC1 using anti-ABP1/AOC1 antibody. ABP1/AOC1 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ABP1/AOC1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of ABP1/AOC1 using anti-ABP1/AOC1 antibody. ABP1/AOC1 was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ABP1/AOC1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of ABP1/AOC1 using anti-ABP1/AOC1 antibody. ABP1/AOC1 was detected in an immunocytochemical section of T-47D cells. The section was incubated with rabbit anti-ABP1/AOC1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Rabbit polyclonal antibody toABP1/AOC1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ABP1 different from the related mouse sequence by ten amino acids, and from the related rat sequence by eight amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AOC1 |
|---|---|
| Gene Synonyms | ABP DAO KAO ABP1 DAO1 KDAO |
| Gene Full Name | amine oxidase copper containing 1 |
| Gene Summary | This gene encodes a metal-binding membrane glycoprotein that oxidatively deaminates putrescine, histamine, and related compounds. The encoded protein is inhibited by amiloride, a diuretic that acts by closing epithelial sodium ion channels. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013] |
| Alternative Names | ABP ABP1 Amiloride binding protein AOC1 DAO DAO1 Diamine oxidase Histaminase KAO Kidney amine oxidase |
| Molecular Weight(MW) | 85 kDa |
| Cellular Localization | Cell membrane,Membrane,Secreted |
WB
Western blot analysis of ABP1/AOC1 using anti-ABP1/AOC1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABP1/AOC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of ABP1/AOC1 using anti-ABP1/AOC1 antibody. ABP1/AOC1 was detected in a paraffin-embedded section of human Colonic adenoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ABP1/AOC1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of ABP1/AOC1 using anti-ABP1/AOC1 antibody. ABP1/AOC1 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ABP1/AOC1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of ABP1/AOC1 using anti-ABP1/AOC1 antibody. ABP1/AOC1 was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ABP1/AOC1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of ABP1/AOC1 using anti-ABP1/AOC1 antibody. ABP1/AOC1 was detected in an immunocytochemical section of T-47D cells. The section was incubated with rabbit anti-ABP1/AOC1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
