WB
Various lysates were subjected to SDS PAGE followed by western blot with ALDOA antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer using ALDOA antibody at dilution of 1:50 (under 10x lens).IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer using ALDOA antibody at dilution of 1:50 (under 10x lens).ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ALDOA antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).IP
IP result of anti-ALDOA (IP:3ug; Detection:1:1000) with HepG2 cells lysate 6000ug.| Product Name | ALDOA Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human ALDOA protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:5000-1:50000 IHC:1:100-1:400 ICC/IF:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ALDOA |
|---|---|
| Gene Synonyms | ALDA GSD12 HEL-S-87p |
| Gene Full Name | aldolase, fructose-bisphosphate A |
| Gene Summary | This gene encodes a member of the class I fructose-bisphosphate aldolase protein family. The encoded protein is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Mutations in this gene have been associated with Glycogen Storage Disease XII, an autosomal recessive disorder associated with hemolytic anemia. Disruption of this gene also plays a role in the progression of multiple types of cancers. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Sep 2017] |
| Alternative Names | Muscle-type aldolase Lung cancer antigen NY-LU-1 Fructose-bisphosphate aldolase A EC:4.1.2.13 Aldolase A |
| Molecular Weight(MW) | 35-40 kDa |
| Cellular Localization | Cytoplasm. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with ALDOA antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer using ALDOA antibody at dilution of 1:50 (under 10x lens).
IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer using ALDOA antibody at dilution of 1:50 (under 10x lens).
ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ALDOA antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).
IP
IP result of anti-ALDOA (IP:3ug; Detection:1:1000) with HepG2 cells lysate 6000ug.| Application Notes | WB:1:5000-1:50000 IHC:1:100-1:400 ICC/IF:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
