WB
Western blot analysis of lysates from EC304 cells (heat-shock treatment), using Phospho-ITK (Tyr512) Antibody at 1/1000 dilution. 5ug/NC membrane strip. Exposure for 10s with ECL Kit. Bands result from membrane strip incubation.IHC
Phospho-ITK (Tyr512) Antibody at 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.IHC
Phospho-ITK (Tyr512) Antibody at 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.IHC
Phospho-ITK (Tyr512) Antibody at 1/200 staining Rat spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.| Product Name | Rabbit polyclonal antibody to Phospho-ITK (Tyr512) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human ITK around the phosphorylation site of Tyr512. |
| Modification | p-Tyr512 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:3000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ITK |
|---|---|
| Gene Synonyms | EMT LYK LPFS1 PSCTK2 |
| Gene Full Name | IL2 inducible T cell kinase |
| Gene Summary | This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008] |
| Alternative Names | EMT Homolog of mouse T cell itk/tsk IL 2 inducible T cell kinase IL2 inducible T cell kinase Interleukin 2 inducible T cell kinase Itk ITK_HUMAN Kinase EMT LPFS1 LYK MGC126257 MGC126258 PSCTK 2 PSCTK2 T cell specific kinase T-cell-specific kinase TSK Tyrosine protein kinase Tyrosine protein kinase ITK/TSK Tyrosine protein kinase Lyk Tyrosine-protein kinase ITK/TSK Tyrosine-protein kinase Lyk |
| Molecular Weight(MW) | 72 kDa(Observed band size: 68 kDa) |
| Cellular Localization | Cytoplasm. Nucleus. |
WB
Western blot analysis of lysates from EC304 cells (heat-shock treatment), using Phospho-ITK (Tyr512) Antibody at 1/1000 dilution. 5ug/NC membrane strip. Exposure for 10s with ECL Kit. Bands result from membrane strip incubation.
IHC
Phospho-ITK (Tyr512) Antibody at 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
IHC
Phospho-ITK (Tyr512) Antibody at 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
IHC
Phospho-ITK (Tyr512) Antibody at 1/200 staining Rat spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.| Application Notes | WB:1:1000-1:3000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
