WB
Western blot analysis of MUC13 using anti-MUC13 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MUC13 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of MUC13 using anti-MUC13 antibody. MUC13 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-MUC13 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of MUC13 using anti-MUC13 antibody. MUC13 was detected in a paraffin-embedded section of mouse colon tissue. The tissue section was incubated with rabbit anti-MUC13 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of MUC13 using anti-MUC13 antibody. MUC13 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-MUC13 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Rabbit polyclonal antibody to MUC13 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human MUC13 recombinant protein (Position: E226-S312). Human MUC13 shares 41.6% amino acid (aa) sequence identity with both mouse and rat MUC13. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MUC13 |
|---|---|
| Gene Synonyms | DRCC1 MUC-13 |
| Gene Full Name | mucin 13, cell surface associated |
| Gene Summary | Epithelial mucins, such as MUC13, are a family of secreted and cell surface glycoproteins expressed by ductal and glandular epithelial tissues (Williams et al., 2001 [PubMed 11278439]).[supplied by OMIM, Jul 2008] |
| Alternative Names | DRCC1 FLJ20063 MUC 13 MUC13 Mucin 13 RECC |
| Molecular Weight(MW) | 55 kDa(Observed band size: 75, 110-120 kDa) |
| Cellular Localization | Cell membrane,Membrane,Secreted |
WB
Western blot analysis of MUC13 using anti-MUC13 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MUC13 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of MUC13 using anti-MUC13 antibody. MUC13 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-MUC13 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of MUC13 using anti-MUC13 antibody. MUC13 was detected in a paraffin-embedded section of mouse colon tissue. The tissue section was incubated with rabbit anti-MUC13 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of MUC13 using anti-MUC13 antibody. MUC13 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-MUC13 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
