IHC
IHC image of REG3A Antibody diluted at 1:500 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.IHC
IHC image of REG3A Antibody diluted at 1:500 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.ICC/IF
Immunofluorescence staining of SH-SY5Y cells with REG3A Antibody at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-REG3A Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Regenerating islet-derived protein 3-alpha protein (27-175AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC |
| IHC:1:500-1:1000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | REG3A |
|---|---|
| Gene Synonyms | HIP PAP PAP1 REG3 INGAP PAP-H PBCGF HIP/PAP REG-III |
| Gene Full Name | regenerating family member 3 alpha |
| Gene Summary | This gene encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. The mature protein also functions as an antimicrobial protein with antibacterial activity. Alternate splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Nov 2014] |
| Alternative Names | FLJ18565 antibody Hepatocarcinoma intestine pancreas antibody Hepatointestinal pancreatic protein antibody HIP antibody HIP/PAP antibody Human proislet peptide antibody INGAP antibody Pancreatic beta cell growth factor antibody Pancreatitis associated protein 1 antibody Pancreatitis associated protein antibody Pancreatitis-associated protein 1 antibody PAP antibody PAP H antibody PAP homologous protein antibody PAP1 antibody PBCGF antibody Proliferation inducing protein 34 antibody Proliferation inducing protein 42 antibody Reg III alpha antibody REG III antibody Reg III-alpha antibody REG-3-alpha antibody REG3 antibody Reg3a antibody REG3A_HUMAN antibody Regenerating islet derived 3 alpha antibody Regenerating islet derived protein 3 alpha precursor antibody Regenerating islet-derived protein 3-alpha 15 kDa form antibody Regenerating islet-derived protein III-alpha antibody |
| Cellular Localization | Secreted. Note=Found in the apical region of pancreatic acinar cells. |
IHC
IHC image of REG3A Antibody diluted at 1:500 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC
IHC image of REG3A Antibody diluted at 1:500 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
ICC/IF
Immunofluorescence staining of SH-SY5Y cells with REG3A Antibody at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | IHC:1:500-1:1000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
