WB
Various lysates were subjected to Tris-Acetate gel system followed by western blot with LAMA1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat colon tissue using LAMA1 antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).