WB
Western blot results show the protein level of Phospho-VEGFR3 (Tyr1230).IHC
Phospho-VEGFR3 (Tyr1230) Antibody at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.ICC/IF
Phospho-VEGFR3 (Tyr1230) Antibody staining A549 cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).| Product Name | Rabbit polyclonal antibody to Phospho-VEGFR3 (Tyr1230) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human VEGFR3 around the phosphorylation site of Tyr1230. |
| Modification | p-Tyr1230 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FLT4 |
|---|---|
| Gene Synonyms | PCL CHTD7 FLT-4 FLT41 LMPH1A LMPHM1 VEGFR3 VEGFR-3 |
| Gene Full Name | fms related receptor tyrosine kinase 4 |
| Gene Summary | This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA. [provided by RefSeq, Jul 2008] |
| Alternative Names | EC 2.7.10.1 flt 4 FLT-4 FLT4 FLT41 Fms related tyrosine kinase 4 Fms-like tyrosine kinase 4 LMPH1A PCL Soluble VEGFR3 variant 1 Soluble VEGFR3 variant 2 Soluble VEGFR3 variant 3 Tyrosine protein kinase receptor FLT4 Tyrosine-protein kinase receptor FLT4 Vascular endothelial growth factor receptor 3 Vascular endothelial growth factor receptor 3 precursor VEGF R3 VEGFR 3 VEGFR-3 VEGFR3 VGFR3_HUMAN |
| Molecular Weight(MW) | 153 kDa |
| Cellular Localization | Cell membrane>Single-pass type I membrane protein. Cytoplasm. Nucleus. |
WB
Western blot results show the protein level of Phospho-VEGFR3 (Tyr1230).
IHC
Phospho-VEGFR3 (Tyr1230) Antibody at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
ICC/IF
Phospho-VEGFR3 (Tyr1230) Antibody staining A549 cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
