WB
Western blot analysis of anti-Porin/VDAC1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Porin/VDAC1 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody. Porin/VDAC1 was detected in a paraffin-embedded section of human lymphadenoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody. Porin/VDAC1 was detected in a paraffin-embedded section of rat heart tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody. Porin/VDAC1 was detected in a paraffin-embedded section of mouse cardiac muscle tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody. Porin/VDAC1 was detected in an immunocytochemical section of Hela cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
IF analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody. Porin/VDAC1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
IF analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody. Porin/VDAC1 was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
IF analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody. Porin/VDAC1 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of Hela cells using anti-Porin/VDAC1 antibody. Overlay histogram showing Hela cells stained with anti-Porin/VDAC1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Porin/VDAC1 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to VDAC1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human Porin/VDAC1 recombinant protein (Position: D78-H181). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VDAC1 |
|---|---|
| Gene Synonyms | PORIN VDAC-1 |
| Gene Full Name | voltage dependent anion channel 1 |
| Gene Summary | This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.[provided by RefSeq, Sep 2010] |
| Alternative Names | hVDAC1 Plasmalemmal porin Porin PORIN 31 HL Porin 31HL Porin 31HM VDAC VDAC 1 VDAC1 VDAC2 VDAC-1 |
| Molecular Weight(MW) | 31 kDa |
| Cellular Localization | Cell membrane,Membrane,Mitochondrion,Mitochondrion outer membrane |
WB
Western blot analysis of anti-Porin/VDAC1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Porin/VDAC1 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody. Porin/VDAC1 was detected in a paraffin-embedded section of human lymphadenoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody. Porin/VDAC1 was detected in a paraffin-embedded section of rat heart tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody. Porin/VDAC1 was detected in a paraffin-embedded section of mouse cardiac muscle tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody. Porin/VDAC1 was detected in an immunocytochemical section of Hela cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
IF analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody. Porin/VDAC1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
IF analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody. Porin/VDAC1 was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
IF analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody. Porin/VDAC1 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of Hela cells using anti-Porin/VDAC1 antibody. Overlay histogram showing Hela cells stained with anti-Porin/VDAC1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Porin/VDAC1 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
