WB
Western blot analysis of GFRA2 using anti-GFRA2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GFRA2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of GFRA2 using anti-GFRA2 antibody. GFRA2 was detected in a paraffin-embedded section of human testis cancer tissue. The tissue section was incubated with rabbit anti-GFRA2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of GFRA2 using anti-GFRA2 antibody. GFRA2 was detected in a paraffin-embedded section of rat testis tissue. The tissue section was incubated with rabbit anti-GFRA2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of GFRA2 using anti-GFRA2 antibody. GFRA2 was detected in a paraffin-embedded section of rat testis tissue. The tissue section was incubated with rabbit anti-GFRA2 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
IF analysis of GFRA2 using anti-GFRA2 antibody. GFRA2 was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-GFRA2 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of HepG2 cells using anti-GFRA2 antibody. Overlay histogram showing HepG2 cells stained with anti-GFRA2 antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GFRA2 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to GFRA2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human GFRA2 recombinant protein (Position: E30-L425). Human GFRA2 shares 97% amino acid (aa) sequence identity with mouse GFRA2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GFRA2 |
|---|---|
| Gene Synonyms | NTNRA RETL2 TRNR2 GDNFRB NRTNR-ALPHA |
| Gene Full Name | GDNF family receptor alpha 2 |
| Gene Summary | Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NTN compared to its other family member, GDNF family receptor alpha 1. This gene is a candidate gene for RET-associated diseases. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] |
| Alternative Names | GDNF family receptor alpha 2 GDNF receptor alpha 2 GDNF receptor beta GDNFR alpha 2 GDNFR beta GDNFRB GFR alpha 2 GFRA2 Neurturin receptor alpha NRTNR ALPHA NTNR alpha NTNRA RET ligand 2 RETL2 TRNR2 |
| Molecular Weight(MW) | 52 kDa |
| Cellular Localization | Cell membrane,Membrane |
WB
Western blot analysis of GFRA2 using anti-GFRA2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GFRA2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of GFRA2 using anti-GFRA2 antibody. GFRA2 was detected in a paraffin-embedded section of human testis cancer tissue. The tissue section was incubated with rabbit anti-GFRA2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of GFRA2 using anti-GFRA2 antibody. GFRA2 was detected in a paraffin-embedded section of rat testis tissue. The tissue section was incubated with rabbit anti-GFRA2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of GFRA2 using anti-GFRA2 antibody. GFRA2 was detected in a paraffin-embedded section of rat testis tissue. The tissue section was incubated with rabbit anti-GFRA2 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
IF analysis of GFRA2 using anti-GFRA2 antibody. GFRA2 was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-GFRA2 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of HepG2 cells using anti-GFRA2 antibody. Overlay histogram showing HepG2 cells stained with anti-GFRA2 antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GFRA2 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
