WB
Western blot analysis of O-GlcNAc-modified proteins in HeLa nuclear extracts (A), A549 whole cell lysates (B), and mouse brain tissue extracts (C).| Product Name | Mouse Monoclonal antibody to O-GlcNAc(HRP Conjugate) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against a peptide containing serine-O-linked N-acetylglucosamine. |
| Conjugation Notes | HRP |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM,k |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:100-1:1000 |
|
| Species Reactivity | All |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Target | O-GlcNAc |
|---|---|
| Function | O-GlcNAc (O-linked N-acetylglucosamine) is a form of protein glycosylation found exclusively in the nucleus and cytoplasm of eukaryotic cells. Many proteins are modified at their serine and threonine hydroxyl groups by the attachment of O-GlcNAc. Proteins that regulate trafficking into and out of the nuclear pore are extensively O-GlcNAcylated. Phosphorylated O-GlcNAc proteins form reversible multimeric complexes with other proteins and these associations are often regulated by phosphorylation. O-GlcNAc proteins may play a key role in pathogenesis of tumors and various cancer cells. O-GlcNAc residues regulate the assembly of the preinitiation complex and are therefore important in transcriptional initiation. Cytoskeletal and membrane O-GlcNAc proteins maintain erythrocyte cell shape and regulate the degradation of proteins responsible for lesions in Alzheimer’s disease. |
WB
Western blot analysis of O-GlcNAc-modified proteins in HeLa nuclear extracts (A), A549 whole cell lysates (B), and mouse brain tissue extracts (C).| Application Notes | WB:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
