WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃ over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cytokeratin 20 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.WB
Western Blot analysis of various cells using Cytokeratin 20 Antibody diluted at 1:500.IHC
Rat colon was stained with anti-Cytokeratin 20 rabbit antibody.IHC
Human urinary bladder transitional carcinoma was stained with anti-Cytokeratin 20 rabbit antibodyICC/IF
Immunofluorescence analysis of HT-29 cell. 1,primary Antibody was diluted at 1:200 (4℃ overnight). 2, Goat Anti Rabbit IgG (H&L) - AFluor 594 Secondary antibody was diluted at 1:500 (room temperature, 50min).| Product Name | Cytokeratin 20 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| IHC:1:200-1:1000 WB:1:2000-1:10000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | KRT20 |
|---|---|
| Gene Synonyms | K20 CD20 CK20 CK-20 KRT21 |
| Gene Full Name | keratin 20 |
| Gene Summary | The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008] |
| Alternative Names | Keratin type I cytoskeletal 20 Cytokeratin-20 CK-20 Keratin-20 K20 Protein IT |
| Molecular Weight(MW) | 49 kDa |
| Cellular Localization | Cytoplasmic , Membranous |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃ over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cytokeratin 20 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
WB
Western Blot analysis of various cells using Cytokeratin 20 Antibody diluted at 1:500.
IHC
Rat colon was stained with anti-Cytokeratin 20 rabbit antibody.
IHC
Human urinary bladder transitional carcinoma was stained with anti-Cytokeratin 20 rabbit antibody
ICC/IF
Immunofluorescence analysis of HT-29 cell. 1,primary Antibody was diluted at 1:200 (4℃ overnight). 2, Goat Anti Rabbit IgG (H&L) - AFluor 594 Secondary antibody was diluted at 1:500 (room temperature, 50min).| Application Notes | IHC:1:200-1:1000 WB:1:2000-1:10000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
