WB
Western blot analysis of anti-ABCB4 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCB4 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of ABCB4 using anti-ABCB4 antibody. ABCB4 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of ABCB4 using anti-ABCB4 antibody. ABCB4 was detected in a paraffin-embedded section of human liver tissue. The tissue section was incubated with rabbit anti-ABCB4 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.FC
Flow Cytometry analysis of HepG2 cells using anti-ABCB4 antibody. Overlay histogram showing HepG2 cells stained with anti-ABCB4 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCB4 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to ABCB4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human ABCB4 recombinant protein (Position: A601-A720). Human ABCB4 shares 79% amino acid (aa) sequence identity with both mouse and rat ABCB4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ABCB4 |
|---|---|
| Gene Synonyms | GBD1 ICP3 MDR2 MDR3 PGY3 ABC21 MDR2/3 PFIC-3 |
| Gene Full Name | ATP binding cassette subfamily B member 4 |
| Gene Summary | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function. [provided by RefSeq, Jul 2008] |
| Alternative Names | ABC21 ABCB4 GBD1 MDR2 MDR2/3 MDR3 Multidrug resistance protein 3 P glycoprotein 3 PFIC 3 PGY3 |
| Molecular Weight(MW) | 142 kDa(Observed band size: 150 kDa) |
| Cellular Localization | Cell membrane,Cytoplasm,Cytoplasmic vesicle,Membrane |
WB
Western blot analysis of anti-ABCB4 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCB4 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of ABCB4 using anti-ABCB4 antibody. ABCB4 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of ABCB4 using anti-ABCB4 antibody. ABCB4 was detected in a paraffin-embedded section of human liver tissue. The tissue section was incubated with rabbit anti-ABCB4 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
FC
Flow Cytometry analysis of HepG2 cells using anti-ABCB4 antibody. Overlay histogram showing HepG2 cells stained with anti-ABCB4 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCB4 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
