WB
Western blot analysis of SNRNP70 using anti-SNRNP70 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SNRNP70 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of SNRNP70 using anti-SNRNP70 antibody. SNRNP70 was detected in a paraffin-embedded section of rat brain(Cerebral cortex) tissue. The tissue section was incubated with rabbit anti-SNRNP70 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of SNRNP70 using anti-SNRNP70 antibody. SNRNP70 was detected in a paraffin-embedded section of rat brain(Cerebral cortex) tissue. The tissue section was incubated with rabbit anti-SNRNP70 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of SNRNP70 using anti-SNRNP70 antibody and anti-Beta Tubulin antibody. SNRNP70 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-SNRNP70 Antibody at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody.FC
Flow Cytometry analysis of HepG2 cells using anti-SNRNP70 antibody. Overlay histogram showing HepG2 cells stained with anti-SNRNP70 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNRNP70 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG(1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to SNRNP70 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human SNRNP70 recombinant protein (Position: M1-D232). Human SNRNP70 shares 100% amino acid (aa) sequence identity with mouse SNRNP70. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SNRNP70 |
|---|---|
| Gene Synonyms | RPU1 Snp1 U1AP U170K U1RNP RNPU1Z SNRP70 U1-70K |
| Gene Full Name | small nuclear ribonucleoprotein U1 subunit 70 |
| Gene Summary | Enables U1 snRNA binding activity. Involved in mRNA splicing, via spliceosome and regulation of RNA splicing. Located in nucleoplasm. Part of U1 snRNP and spliceosomal complex. Implicated in disease of mental health and systemic lupus erythematosus. Biomarker of Alzheimer's disease. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | SNRNP70 RNPU1Z RPU1 SNRP70 U1AP1 U1 small nuclear ribonucleoprotein 70 kDa U1 snRNP 70 kDa U1-70K snRNP70 |
| Molecular Weight(MW) | 52 kDa(Observed band size: 52, 63 kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of SNRNP70 using anti-SNRNP70 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SNRNP70 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of SNRNP70 using anti-SNRNP70 antibody. SNRNP70 was detected in a paraffin-embedded section of rat brain(Cerebral cortex) tissue. The tissue section was incubated with rabbit anti-SNRNP70 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of SNRNP70 using anti-SNRNP70 antibody. SNRNP70 was detected in a paraffin-embedded section of rat brain(Cerebral cortex) tissue. The tissue section was incubated with rabbit anti-SNRNP70 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of SNRNP70 using anti-SNRNP70 antibody and anti-Beta Tubulin antibody. SNRNP70 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-SNRNP70 Antibody at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody.
FC
Flow Cytometry analysis of HepG2 cells using anti-SNRNP70 antibody. Overlay histogram showing HepG2 cells stained with anti-SNRNP70 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNRNP70 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG(1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
