WB
Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000.| Product Name | LATS1/2 (Phospho Thr1079/1041) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthesized phospho derived from human LATS1/2 (Phospho-Thr1079/1041) |
| Modification | p-Thr1079/1041 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LATS1 |
|---|---|
| Gene Synonyms | wts WARTS |
| Gene Full Name | large tumor suppressor kinase 1 |
| Gene Summary | The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. [provided by RefSeq, Apr 2017] |
| Alternative Names | Serine/threonine-protein kinase LATS1 Large tumor suppressor homolog 1 WARTS protein kinase h-warts |
| Molecular Weight(MW) | 140 kDa |
| Cellular Localization | Cytoplasm , cytoskeleton , microtubule organizing center , centrosome . Cytoplasm , cytoskeleton , spindle . Midbody . Cytoplasm , cytoskeleton , microtubule organizing center , spindle pole body . Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus , including spindle pole bodies , mitotic spindle , and midbody , during mitosis. |
WB
Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
