WB
Western blot analysis of Histone H3 (citrulline R2) on different lysates with Rabbit anti-Histone H3 (citrulline R2) antibody at 1/2,000 dilution. Lane 1: 293T (Human embryonic kidney cells) cells transfected with PADI4 Lane 2: 293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours Lysates/proteins at 20 µg/Lane. Exposure time: 4 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: Rabbit anti-Histone H3 (citrulline R2) antibody, 1/2,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.IP
Immunoprecipitation (IP): Histone H3 (citrulline R2) was immunoprecipitated in 0.2 mg 293T (Human embryonic kidney cells) cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours cell lysate with Rabbit anti-Histone H3 (citrulline R2) antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Histone H3 (citrulline R2) antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours cell lysate (input) Lane 2: Rabbit anti-Histone H3 (citrulline R2) antibody IP in 293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours cell lysate Lane 3: Rabbit IgG instead of Rabbit anti-Histone H3 (citrulline R2) antibody in 293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours cell lysate Exposure time: 2 minutes Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: Rabbit anti-Histone H3 (citrulline R2) antibody, 1/1,000 in primary antibody dilution buffer, 2 hours at room temperature| Product Name | Histone H3 (citrulline R2) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB:1:2000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | H3C1 |
|---|---|
| Gene Synonyms | H3/A H3FA HIST1H3A |
| Gene Full Name | H3 clustered histone 1 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 15 kDa |
| Cellular Localization | Nucleus, Chromosome. |
| SwissProt ID | P68431 |
|---|
WB
Western blot analysis of Histone H3 (citrulline R2) on different lysates with Rabbit anti-Histone H3 (citrulline R2) antibody at 1/2,000 dilution. Lane 1: 293T (Human embryonic kidney cells) cells transfected with PADI4 Lane 2: 293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours Lysates/proteins at 20 µg/Lane. Exposure time: 4 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: Rabbit anti-Histone H3 (citrulline R2) antibody, 1/2,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
IP
Immunoprecipitation (IP): Histone H3 (citrulline R2) was immunoprecipitated in 0.2 mg 293T (Human embryonic kidney cells) cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours cell lysate with Rabbit anti-Histone H3 (citrulline R2) antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Histone H3 (citrulline R2) antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours cell lysate (input) Lane 2: Rabbit anti-Histone H3 (citrulline R2) antibody IP in 293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours cell lysate Lane 3: Rabbit IgG instead of Rabbit anti-Histone H3 (citrulline R2) antibody in 293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours cell lysate Exposure time: 2 minutes Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: Rabbit anti-Histone H3 (citrulline R2) antibody, 1/1,000 in primary antibody dilution buffer, 2 hours at room temperature| Application Notes | WB:1:2000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
