WB
Western blot analysis of various lysates using DiMethyl-Histone H3-K79 Rabbit mAb at 1:65000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Negative control (NC): H3 protein Exposure time: 90s.DB
Dot-blot analysis of all sorts of peptides using DiMethyl-Histone H3-K79 antibody at 1:20000 dilution.IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using DiMethyl-Histone H3-K79 Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using DiMethyl-Histone H3-K79 Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using DiMethyl-Histone H3-K79 Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.ICC/IF
Confocal imaging of NIH/3T3 cells using DiMethyl-Histone H3-K79 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.IF-P
Confocal imaging of paraffin-embedded Human spleen tissue using DiMethyl-Histone H3-K79 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.IP
Immunoprecipitation analysis of 300 μg extracts of 293F cells using 3 μg DiMethyl-Histone H3-K79 antibody. Western blot was performed from the immunoprecipitate using DiMethyl-Histone H3-K79 antibody at a dilution of 1:20000.CHIP
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using DiMethyl-Histone H3-K79 Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.CHIP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 293T cells and DiMethyl-Histone H3-K79 Rabbit mAb. The ChIP sequencing results indicate the enrichment pattern of DiMethyl-Histone H3-K79 in selected genomic region and representative gene loci (GAPDH), as shown in figure.| Product Name | DiMethyl-Histone H3-K79 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic dimethylated peptide around K79 of human Histone H3-K79 (NP_003520.1). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPDBICC/IFIF-PIHCIPWB |
| WB:1:30000-1:120000 IP: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ICC/IF:1:200-1:400 IF-P:1:50-1:200 IHC:1:2000-1:10000 DB:1:2000-1:20000 ChIP: 5μg antibody for 5μg-10μg of Chromatin |
|
| Species Reactivity | HumanMouseOtherRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3-4 |
|---|---|
| Gene Synonyms | H3t H3.4 H3/g H3FT H3C16 HIST3H3 |
| Gene Full Name | H3.4 histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 15 kDa(Observed band size: 17 kDa) |
| Cellular Localization | Chromosome, Nucleus. |
| SwissProt ID | P68431 |
|---|
WB
Western blot analysis of various lysates using DiMethyl-Histone H3-K79 Rabbit mAb at 1:65000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Negative control (NC): H3 protein Exposure time: 90s.
DB
Dot-blot analysis of all sorts of peptides using DiMethyl-Histone H3-K79 antibody at 1:20000 dilution.
IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using DiMethyl-Histone H3-K79 Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using DiMethyl-Histone H3-K79 Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using DiMethyl-Histone H3-K79 Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ICC/IF
Confocal imaging of NIH/3T3 cells using DiMethyl-Histone H3-K79 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
IF-P
Confocal imaging of paraffin-embedded Human spleen tissue using DiMethyl-Histone H3-K79 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
IP
Immunoprecipitation analysis of 300 μg extracts of 293F cells using 3 μg DiMethyl-Histone H3-K79 antibody. Western blot was performed from the immunoprecipitate using DiMethyl-Histone H3-K79 antibody at a dilution of 1:20000.
CHIP
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using DiMethyl-Histone H3-K79 Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
CHIP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 293T cells and DiMethyl-Histone H3-K79 Rabbit mAb. The ChIP sequencing results indicate the enrichment pattern of DiMethyl-Histone H3-K79 in selected genomic region and representative gene loci (GAPDH), as shown in figure.| Application Notes | WB:1:30000-1:120000 IP: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ICC/IF:1:200-1:400 IF-P:1:50-1:200 IHC:1:2000-1:10000 DB:1:2000-1:20000 ChIP: 5μg antibody for 5μg-10μg of Chromatin |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
