WB
Western blot analysis of various lysates using TriMethyl-Histone H3-K9 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): H3 protein. Exposure time: 45 s.DB
Dot-blot analysis of all sorts of peptides using TriMethyl-Histone H3-K9 Rabbit mAb at 1:1000 dilution.ICC/IF
Confocal imaging of HeLa cells using TriMethyl-Histone H3-K9 Rabbit mAb (dilution 1:400) followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.CHIP
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K9 Rabbit mAb and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.CHIP
Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells, using TriMethyl-Histone H3-K9 Rabbit mAb. The ChIP sequencing results indicate the enrichment pattern of TriMethyl-Histone H3-K9 in selected genomic region and representative gene loci (ZNF404,GAPDH), as shown in figure.CUT&Tag
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ K562 cells with 1 μg TriMethyl-Histone H3-K9 Rabbit mAb, along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of TriMethyl-Histone H3-K9 in representative gene loci (MYT1), as shown in figure.| Product Name | TriMethyl-Histone H3-K9 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPDBICC/IFWBCUT&Tag-seq |
| WB:1:1000-1:4000 DB:1:1000-1:2000 ICC/IF:1:200-1:800 ChIP:5μg antibody for 5μg-10μg of Chromatin CUT&Tag:10⁵ cells /1 μg |
|
| Species Reactivity | HumanMouseOtherRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3-4 |
|---|---|
| Gene Synonyms | H3t H3.4 H3/g H3FT H3C16 HIST3H3 |
| Gene Full Name | H3.4 histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 15 kDa(Observed band size: 17 kDa) |
| Cellular Localization | Chromosome, Nucleus. |
| SwissProt ID | P68431 |
|---|
WB
Western blot analysis of various lysates using TriMethyl-Histone H3-K9 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): H3 protein. Exposure time: 45 s.
DB
Dot-blot analysis of all sorts of peptides using TriMethyl-Histone H3-K9 Rabbit mAb at 1:1000 dilution.
ICC/IF
Confocal imaging of HeLa cells using TriMethyl-Histone H3-K9 Rabbit mAb (dilution 1:400) followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
CHIP
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K9 Rabbit mAb and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
CHIP
Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells, using TriMethyl-Histone H3-K9 Rabbit mAb. The ChIP sequencing results indicate the enrichment pattern of TriMethyl-Histone H3-K9 in selected genomic region and representative gene loci (ZNF404,GAPDH), as shown in figure.
CUT&Tag
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ K562 cells with 1 μg TriMethyl-Histone H3-K9 Rabbit mAb, along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of TriMethyl-Histone H3-K9 in representative gene loci (MYT1), as shown in figure.| Application Notes | WB:1:1000-1:4000 DB:1:1000-1:2000 ICC/IF:1:200-1:800 ChIP:5μg antibody for 5μg-10μg of Chromatin CUT&Tag:10⁵ cells /1 μg |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
