WB
Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H4-K5 Rabbit mAb at 1:10000 dilution. HeLa cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.WB
Western blot analysis of lysates from C6 cells, using Acetyl-Histone H4-K5 Rabbit mAb at 1:10000 dilution. C6 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.db
Dot-blot analysis of all sorts of peptides using Acetyl-Histone H4-K5 Rabbit mAb at 1:1000 dilution.IHC
Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Acetyl-Histone H4-K5 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Acetyl-Histone H4-K5 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Acetyl-Histone H4-K5 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.IP
Immunoprecipitation of Acetyl-Histone H4-K5 from 600 µg extracts of HeLa cells treated with TSA(1 μM,18h) was performed using 5 µg of Acetyl-Histone H4-K5 Rabbit mAb. Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1x Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Acetyl-Histone H4-K5 Rabbit mAb at a dilution of 1:10000.ChIP
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H4-K5 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.ChIP-seq
Chromatin immunoprecipitations were performed with cross-linked chromatin from Hela cells and Acetyl-Histone H4-K5 Rabbit mAb. The ChIP sequencing results indicate the enrichment pattern of Acetyl-Histone H4-K5 in selected genomic region and representative gene loci (GAPDH), as shown in figure.CUT&Tag
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ K562 cells with 1 μg Acetyl-Histone H4-K5 Rabbit mAb, along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of Acetyl-Histone H4-K5 in representative gene loci (GAPDH), as shown in figure.| Product Name | Acetyl-Histone H4-K5 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPDBIHCIPWBCUT&Tag-seq |
| WB:1:2000-1:10000 IP:0.5 μg-5 μg antibody for 400 μg-600 μg extracts of whole cells IHC:1:500-1:1000 DB:1:500-1:2000 ChIP:5 μg antibody for 5 μg-10 μg of Chromatin ChIP-seq:1:50-1:200 CUT&Tag:10⁵cells/1μg |
|
| Species Reactivity | HumanMouseOtherRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H4C1 |
|---|---|
| Gene Synonyms | H4FA HIST1H4A |
| Gene Full Name | H4 clustered histone 1 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 11 kDa(Observed band size: 11 kDa/14 kDa) |
| Cellular Localization | Chromosome, nucleus. |
| SwissProt ID | P62805 |
|---|
WB
Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H4-K5 Rabbit mAb at 1:10000 dilution. HeLa cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
WB
Western blot analysis of lysates from C6 cells, using Acetyl-Histone H4-K5 Rabbit mAb at 1:10000 dilution. C6 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
db
Dot-blot analysis of all sorts of peptides using Acetyl-Histone H4-K5 Rabbit mAb at 1:1000 dilution.
IHC
Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Acetyl-Histone H4-K5 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Acetyl-Histone H4-K5 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Acetyl-Histone H4-K5 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
IP
Immunoprecipitation of Acetyl-Histone H4-K5 from 600 µg extracts of HeLa cells treated with TSA(1 μM,18h) was performed using 5 µg of Acetyl-Histone H4-K5 Rabbit mAb. Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1x Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Acetyl-Histone H4-K5 Rabbit mAb at a dilution of 1:10000.
ChIP
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H4-K5 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
ChIP-seq
Chromatin immunoprecipitations were performed with cross-linked chromatin from Hela cells and Acetyl-Histone H4-K5 Rabbit mAb. The ChIP sequencing results indicate the enrichment pattern of Acetyl-Histone H4-K5 in selected genomic region and representative gene loci (GAPDH), as shown in figure.
CUT&Tag
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ K562 cells with 1 μg Acetyl-Histone H4-K5 Rabbit mAb, along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of Acetyl-Histone H4-K5 in representative gene loci (GAPDH), as shown in figure.| Application Notes | WB:1:2000-1:10000 IP:0.5 μg-5 μg antibody for 400 μg-600 μg extracts of whole cells IHC:1:500-1:1000 DB:1:500-1:2000 ChIP:5 μg antibody for 5 μg-10 μg of Chromatin ChIP-seq:1:50-1:200 CUT&Tag:10⁵cells/1μg |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
