WB
Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H3-K4 Rabbit mAb at 1:1000 dilution. HeLa cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 20s.DB
Dot-blot analysis of all sorts of peptides using Acetyl-Histone H3-K4 Rabbit mAb at 1:1000 dilution.IHC
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Acetyl-Histone H3-K4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Mouse testis using Acetyl-Histone H3-K4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Rat colon using Acetyl-Histone H3-K4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.ICC/IF
Confocal imaging of HeLa cells (treated with TSA) and HeLa cells (untreated) using Acetyl-Histone H3-K4 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.IP
Immunoprecipitation of Acetyl-Histone H3-K4 from 600 µg extracts of HeLa cells treated with TSA (5mM ,16h) was performed using 3 µg of Acetyl-Histone H3-K4 Rabbit mAb. Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Acetyl-Histone H3-K4 Rabbit mAb at a dilution of 1:500.CHIP
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K4 Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.| Product Name | Acetyl-Histone H3-K4 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPDBICC/IFIHCIPWB |
| WB:1:1000-1:3000 IP: 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells ICC/IF:1:200-1:2000 IHC:1:50-1:200 DB:1:1000-1:5000 ChIP:5μg antibody for 5μg-10μg of Chromatin |
|
| Species Reactivity | HumanMouseOtherRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3-4 |
|---|---|
| Gene Synonyms | H3t H3.4 H3/g H3FT H3C16 HIST3H3 |
| Gene Full Name | H3.4 histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 15 kDa(Observed band size: 17 kDa) |
| Cellular Localization | Chromosome, Nucleus. |
| SwissProt ID | P68431 |
|---|
WB
Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H3-K4 Rabbit mAb at 1:1000 dilution. HeLa cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 20s.
DB
Dot-blot analysis of all sorts of peptides using Acetyl-Histone H3-K4 Rabbit mAb at 1:1000 dilution.
IHC
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Acetyl-Histone H3-K4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Mouse testis using Acetyl-Histone H3-K4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Rat colon using Acetyl-Histone H3-K4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
ICC/IF
Confocal imaging of HeLa cells (treated with TSA) and HeLa cells (untreated) using Acetyl-Histone H3-K4 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
IP
Immunoprecipitation of Acetyl-Histone H3-K4 from 600 µg extracts of HeLa cells treated with TSA (5mM ,16h) was performed using 3 µg of Acetyl-Histone H3-K4 Rabbit mAb. Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Acetyl-Histone H3-K4 Rabbit mAb at a dilution of 1:500.
CHIP
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K4 Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.| Application Notes | WB:1:1000-1:3000 IP: 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells ICC/IF:1:200-1:2000 IHC:1:50-1:200 DB:1:1000-1:5000 ChIP:5μg antibody for 5μg-10μg of Chromatin |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
